【摘要】 目的以苯并(a)芘诱导小鼠前胃癌模型,研究番茄红素对前胃癌小鼠氧化损伤的保护作用。方法将120只昆明种小鼠随机分为4组:正常对照组、损伤组[苯并(a)芘组]、番茄红素高剂量组[20 mg/kg+苯并(a)芘]、番茄红素低剂量组[5 mg/kg+苯并(a)芘]。番茄红素组与损伤组给予苯并(a)芘,建立前胃癌模型,观察不同浓度的番茄红素对小鼠前胃癌形成的作用。24周后,观察小鼠前胃癌形成情况,检测血清及肝匀浆中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)的含量,同时检测淋巴细胞DNA氧化损伤情况。结果番茄红素具有明显的抗肿瘤作用,给予番茄红素后能降低小鼠前胃癌肿瘤直径[模型组(0.33±0.20)cm,番茄红素高剂量+苯并(a)芘组(0.12±0.04)cm],提高小鼠血清和肝匀浆中SOD活力[损伤组血清中为(47.18±3.27)U/ml,肝匀浆中为(39.83±6.63)U/mg.Pro;番茄红素高剂量组血清中为(67.27±9.38)U/ml,肝匀浆中为(54.49±4.86)U/mg.Pro]、GSH-Px活力[损伤组血清中为(170.63±15.25)U/ml,肝匀浆中为(54.60±5.19)U/mg.Pro;番茄红素高剂量组血清中为(184.22±13.81)U/ml.Pro,肝匀浆中为(66.78±11.97)U/mg.Pro],降低MDA含量[模型组血清中为(11.08±1.82)nmol/mg,肝匀浆中为(12.47±2.62)nmol/mg.Pro;番茄红素高剂量组血清(7.63±0.85)nmol/mg,肝匀浆(8.28±1.74)nmol/mg.Pro]和减少淋巴细胞DNA的氧化损伤的作用,差异均有统计学意义(P<0.05)。结论番茄红素对苯并(a)芘诱导的小鼠前胃癌具有一定的抑制作用,对细胞DNA氧化损伤具有明显的保护作用。更多还原

【Abstract】 [Objective] Using the benzo(a)pyrene-induced forestomach carcinoma model in mice,to study the protective effect of lycopene on the oxidative damage of forestomach carcinoma in mice.[Methods]120 Kunming mice were randomly and equally divided into 4 groups: normal group,injury group [B(a)P group],high-dose lycopene group [20 mg/kg + B(a)P ] and low-dose lycopene group [5mg/kg + B(a)P].The lycopene group and the injury group were given the B(a)P to establish the forestomach carcinoma model,and observe the effect of different concentrations of lycopene on the formation of forestomach carcinoma in mice.After 24 weeks,the levels of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) and malondialdehyde(MDA) in serum and liver homogenate were detected,as well as the DNA oxidative damage in lymphocytes was tested.[Results]Lycopene showed strongly inhibitory effect on forestomach tumor formation.It reduced the diameter of forestomach carcinoma [(0.33±0.20) cm in the model group and(0.12±0.04) cm in high-dose lycopene group],increased the activities of SOD[serum(47.18±3.27)U/ml and liver homogenate(39.83±6.63) U/mg·Pro in the injury group,serum(67.27±9.38) U/ml and liver homogenate(54.49±4.86) U/mg·Pro in high-dose lycopene group]and GSH-Px [serum(170.63±15.25) U/ml and liver homogenate(54.60±5.19) U/mg·Pro in the injury group,serum(184.22±13.81) U/ml and liver homogenate(66.78±11.97) U/mg·Pro in high-dose lycopene group],reduced the level of MDA [ serum(11.08±1.82) nmol/ml and liver homogenate(12.47±2.62) nmol/mg·Pro in the model group,serum(7.63±0.85) nmol/ml and liver homogenate(8.28±1.74) nmol/mg·Pro in high-dose lycopene group],and decreased the DNA oxidative damage in lymphocytes(all P<0.05).[Conclusion]Lycopene can inhibit B(a)P-induced forestomach carcinoma model in mice,and has a protective effect on DNA oxidative damage in cells.更多还原