【摘要】 目的:改进天麻中天麻素提取及测定方法。方法:利用热回流提取法,并采用HPLC进行检测,在单因素实验的基础上,利用响应面分析法确定天麻素最佳提取条件。结果:以纯净水为溶剂,在沸水浴条件下,料液比1∶37,提取2次,每次126 min,天麻素的含量达0.377%。此结果比2010年版中国药典天麻素提取方法所得天麻素含量提高了2.12倍。结论:热水回流提取法天麻素的溶出率高,可更准确的反映天麻中天麻素的真实含量。更多还原

【Abstract】 Objective:To improve an extraction and determination method for gastrodin in Gastrodia elata.Methods:On the basis of single factor experiment,used the method of hot water reflux extraction and applied the response surface methodology to determine the best conditions for extracting gastrodin and using HPLC test.Results:Under the boiling water condition,pure water as solvent,the solid-liquid ratio was 1∶ 37,extraction 2 times and extraction time 126 min per times,the content of gastrodin was 0.377%.Comparison with the 2010 edition of the China pharmacopoeia of gastrodin extracted in Gastrodia elata,the result showed the gastrodin content was increased by 2.12 times.Conclusion:This extraction method of gastrodin dissolution rate is high and can reflect the real content of gastrodin of Gastrodia elata.更多还原