【摘要】 [目的]探讨复方中药清肠栓对溃疡性结肠炎(UC)大鼠结肠黏膜纤丝状肌动(F-actin)蛋白的修复作用。[方法]清洁级雄性SD大鼠36只,随机分为正常组,模型组,柳氮磺胺吡啶(SASP)组,清肠栓高、中、低剂量组,每组6只。选用三硝基苯磺酸(TNBS)诱导的UC大鼠模型,采用免疫荧光的方法观察各组大鼠结肠黏膜F-actin蛋白的表达,并运用图像分析软件进行平均光密度测定,观察清肠栓对UC大鼠结肠屏障的修复作用。[结果]UC发病对结肠黏膜细胞骨架系统严重损害,模型组大鼠结肠黏膜F-actin蛋白几乎完全被破坏,荧光染色暗淡。图像分析测其平均光密度较正常组明显降低(P<0.01)。清肠栓能不同程度减轻UC黏膜的破坏,改善F-actin蛋白的分布,并使其表达升高(P<0.01)。[结论]清肠栓能有效地调节肠黏膜屏障功能,有效抑制UC大鼠结肠通透性的升高,改善肠黏膜屏障功能,促进溃疡愈合。更多还原

【Abstract】 [Objective]To observe the effect of Qingchang Suppository(QS)on filamentous actin(F-actin) of colon in an experimental rat model of ulcerative colitis(UC).[Methods]Thirty-six SD rats were randomly divided into 6 equal groups: normal control group,model group,SASP solution group,QS groups of high,medium and low doses.The rat model of ulcerative colitis was induced by trinitro-benzene-sulfonic acid(TNBS).The modulatory effect of QS on UC was examined by immunofluorescent labeling of F-actin in rat colonic mucosa.[Results]UC caused severe damage of the cytoskeleton system in colonic mucosa,which was showed by disruption of F-actin.Compared to normal control group,the expression of F-actin was significantly lower in model group(P<0.01) Treatment with QS attenuated the disruption of F actin(P<0.01).[Conclusion]This study demonstrated that QS can effectively modulate intestinal mucosal barrier function and inhibit colonic permeability,thus improve ulcer healing.更多还原