【摘要】 目的观察钼酸氨对体外培养人肝癌细胞的增殖抑制和诱导凋亡的作用。方法应用CCK-8法检测不同浓度钼酸氨对HepG2细胞的增殖抑制作用;应用流式细胞仪检测细胞周期分布及细胞凋亡率。结果钼酸氨能明显抑制HepG2细胞增殖、诱导细胞凋亡、影响细胞周期,并存在剂量-效应关系和时间-效应关系,随着钼酸氨浓度升高和作用时间延长,凋亡细胞和G0/G1期细胞比例逐渐增加,S期及G2/M期细胞减少,并出现凋亡峰。结论钼酸氨能抑制HepG2细胞增殖,诱导细胞凋亡,影响细胞周期,将细胞特异性地阻滞在G1期。更多还原

【Abstract】 【Objective】To investigate the effect of ammoniummolybdate on inhibiting proliferation and inducing apoptosis of liver cancer cell.【Methods】The effect of different Ammoniummolybdate concentration on Hep G2 cell proliferation was examined using CCK8.The rate of cells’ apoptosis was detected by flow cytometer(FCM).【Results】 Ammoniummolybdate can obviously inhibit Hep G2 cell proliferation,and induce apoptosis of liver cancer cells,and effect the cells cycle.The relation of dosage and time to domino offect exists.The proportion of apoptosis and G0/G1 period cells rose,S period cells and G2/M period cells decreased gradually with the increase of ammoniummolybdate concentration and the protraction of action time.The cells were especially retarded in G0/G1 period and apoptosis apex occurs.【Conclusion】Ammoniummolybdate can obviously inhibit HepG2 cell proliferation,and induce apoptosis of liver cancer cells,and change the cells cycle specially.更多还原