【摘要】 目的研究氯沙坦和卡托普利对腹主动脉瘤(AAA)形成的抑制作用。方法雄性Wistar大鼠30只,体质量250～300 g,随机分为对照组、氯沙坦组和卡托普利组,每组10只,以腔内猪胰弹力蛋白酶灌注法复制AAA动物模型,术后分别给予生理盐水、氯沙坦及卡托普利灌胃,观察各组AAA形成情况,并用HE染色及VVG染色检测主动脉壁内炎症浸润及中膜弹力纤维破坏情况,用免疫组织化学染色检测主动脉壁内MMP-2和MMP-9的表达情况。结果对照组均形成AAA;而氯沙坦组和卡托普利组均未形成AAA,与对照组相比,氯沙坦组和卡托普利组腹主动脉壁增厚不明显,中膜弹力纤维层及平滑肌细胞保存较好,炎症细胞浸润明显减少,主动脉壁内MMP-2和MMP-9的表达均较对照组明显减少;而氯沙坦组和卡托普利组之间差异无统计学意义。结论氯沙坦和卡托普利能抑制弹力蛋白酶灌注的大鼠主动脉壁的炎症反应和主动脉壁的MMP的表达,最终抑制弹力蛋白酶灌注大鼠AAA的形成。为证实血管紧张素Ⅱ在AAA发病机制中的作用和小AAA的药物治疗提供了理论基础。更多还原

【Abstract】 【Objective】 To investigate the effects of Losartan and Captopril on the experimental abdominal aortic aneurysm(AAA) formation in rat model.【Methods】 Thirty male Wistar rats(250~300 g) were divided into three groups,each group contents ten rats,all animals underwent aortic perfusion with elastase to set up AAA model.The rats of experimental groups were given Losartan or Captopril as well as control group were given saline by gastric irrigation after operation.The expansion of abdominal aorta and the histological changes of the aorta of three groups were examined and the expression of MMP-2 and MMP-9 was detected by immunohistochemical analysis.【Results】 Notably,the expansion of abdominal aorta was inhibited in rats treated with Losartan or Captopril(P <0.01).There is a notable reduction of inflammatory response in experimental group(P <0.01),and consistent with it,the expression of MMP-2,MMP-9 was remarkable reduced(P <0.01).The difference between Losartan and Captopril group was not statistically significant(P >0.05).【Conclusions】 Both Losartan and Captopril can suppress the inflammatory response and the expression of MMP-2 and MMP-9 in the aortic wall,thereby inhibit the formation of experimental AAA in the rats.更多还原