【摘要】 目的研究二十二碳六烯酸(docosahexaenoic acid,DHA)和花生四烯酸(arachidonic acid,AA)对人胆囊癌细胞GBC-SD增殖的影响,通过相关蛋白质指标,探讨可能的分子机制。方法 MTT法检测不同浓度(10~80 ug/mL)DHA和AA对胆囊癌细胞GBC-SD存活率的影响;免疫印迹检测β-catenin、c-myc、cyclinD1三种蛋白表达量的变化。结果当DHA浓度达到60 ug/mL时可明显抑制胆囊癌细胞GBC-SD存活并随着浓度升高抑制作用增强,呈剂量依赖(P<0.05),而相同浓度AA无明显作用。同时β-catenin、c-myc、cyclinD1三种蛋白表达量均下降。结论 DHA可抑制人胆囊癌细胞GBC-SD增殖,使β-catenin、c-myc、cyclin D1表达减少。更多还原

【Abstract】 【Objective】 To investigate the effect of DHA and AA on human carcinoma of gallbladder cell line cells GBC-SD in vitro and explore possible molecular mechanisms through the relevant protein targets.【Methods】 GBC-SD cell viability is examined for different concentration(10～80 ug/Ml) of DHA and AA by MTT assay.Protein expression of β-catenin,c-myc,cyclin D1 was measured by Western-blot.【Results】 The DHA showed a dose dependent inhibition to the survival of GBC-SD after the concentration above 60 ug/mL(P <0.05).However,there is no significant effect for AA treatment group.The expression of protein β-catenin,c-myc,cyclin D1 are all decreased by western-blot.【Conclusion】 DHA can effectively inhabit the proliferation of human carcinoma of gallbladder cell line cells GBC-SD,decrease the expression of protein β-catenin,c-myc,cyclin D1.更多还原