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小偃6号及其衍生后代品质相关性状基因的分子检测

Molecular Detection of Quality Traits-related Genes in Wheat Cultivar Xiaoyan 6 and Its Derivatives

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【作者】 陈桂玲余利王黎明李滨童依平王洪刚李兴锋

【Author】 CHEN Gui-ling1,YU Li1,WANG Li-ming2,LI Bin3,TONG Yi-ping3,WANG Hong-gang1,LI Xing-feng 1 (1State Key Laboratory of Crop Biology/ Shandong Key Laboratory of Crop Biology/ Shandong Agricultural University,Taian 271018;2Agronomy College,Henan University of Science and Technology,Luoyang 471003;3Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101)

【机构】 山东农业大学/山东省作物生物学重点实验室/作物生物学国家重点实验室河南科技大学农学院中国科学院遗传与发育生物学研究所

【摘要】 小偃6号是我国小麦育种的重要骨干亲本之一。本研究利用UMN19、UMN25、UMN26、T2、T5、T13、S13、S1、T1、Wx-A1、Wx-B1、Wx-D1、YP7A、YP7B-1、PPO18、PPO29等16个功能型分子标记对小偃6号及其衍生后代的品质相关性状的基因组成进行了检测和分析。结果表明:在高分子量谷蛋白亚基两位点(Glu-A1、Glu-D1)和Waxy蛋白基因位点上,分别有78.72%、82.98%的衍生品种与骨干亲本小偃6号等位基因一致,但少数品种具有不同的优良亚基等位基因Ax2*或Dx5+Dy10;在低分子量谷蛋白亚基两位点(Glu-B3、Glu-D3)上,有25.33%的衍生品种与小偃6号等位基因一致;在八氢番茄红素合成酶基因位点Psy-A1、Psy-B1和多酚氧化酶(PPO)活性等位基因位点上,仅有17.02%和38.29%的衍生品种与小偃6号一致;并探讨了不同品质相关性状基因位点在衍生后代传递频率存在差异的原因。

【Abstract】 Wheat cultivar Xiaoyan 6 is an important foundation parent in wheat improvement of China.Sixteen functional molecular markers including UMN19,UMN25,UMN26,T2,T5,T13,S13,S1,T1,Wx-A1,Wx-B1,Wx-D1,YP7A,YP7B-1,PPO18,and PPO29 were used to identify the composition of genes associated with quality traits in Xiaoyan 6 and its derivatives.Results showed that 78.72% and 82.98% derivatives had the same compositions of genes as Xiaoyan 6 at loci Glu-A1 and Glu-D1 of HMW-GS and locus Wx.Some derivative cultivars had different HMW-GS gene composition,such as Ax2* and Dx5+Dy10,and 25.33% derivatives had the same gene compositions as Xiaoyan 6 at loci Glu-B3 and Glu-D3.Only 17.02% and 38.29% derivative cultivars had the same gene compositions as Xiaoyan 6 at phytoene synthase(PSY) loci Psy-A1 and Psy-B1 and(PPO) gene.The possible reason of the different transmission frequency of these loci in Xiaoyan 6 and its derivatives were discussed.

  • 【文献出处】 植物遗传资源学报 ,Journal of Plant Genetic Resources , 编辑部邮箱 ,2012年03期
  • 【分类号】S512.1
  • 【被引频次】9
  • 【下载频次】256
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