【摘要】 感染病毒的香石竹组培苗于(38.5±1)℃条件下热处理29d后,切取0.1～0.2mm的茎尖生长点无菌培养成苗后,经DAS-Elisa检测获得脱除香石竹斑驳病毒(Carnation mottle virus,CarMV)的香石竹脱毒苗,取脱毒苗与非脱毒苗的叶片,测量叶绿素a、叶绿素b、类胡萝卜素、丙二醛(MDA)、干物质含量几种生理生化指标。结果表明,脱毒苗与非脱毒苗相比:(1)叶绿素和类胡萝卜素含量增加,具有较强的光合效率;(2)与抗性生理有关的MDA含量下降,衰老减缓;(3)光合物质的形成与积累增多,干物质含量增加。更多还原

【Abstract】 The common tissue culture seedlings were cultured in heat-treatment conditions(38.5±1) oC for 29 d,then the stem-apexes were cut at 0.1-0.2 mm and cultured in sterile condition.Carnation mottle virus(CarMV)free carnation plants were obtained which had been detected CarMV by DAS-Elisa.Leaves of CarMV-free seedlings and common seedlings were cut down and measured the contents of chlorophyll a,chlorophyll b,carotenoids,malondialdehyde(MDA) and dry-matter.The results showed that comparative study between CarMV-free seedlings and common seedlings:(1) chlorophyll and carotenoids content of CarMV-free seedlings increased,and the photosynthetic efficiency was high;(2) MDA content which relate to the physiological resistance of CarMV-free seedlings decreased,delaying aging;(3) formation and accumulation of photosynthates increased that could cause more dry-matter content.更多还原