【摘要】 目的:探讨珠江水中O1/O139群霍乱弧菌检出率与珠江水体中理化因素之间的相关性,为霍乱的防控工作提供参考。方法:2010年每月在珠江河沿岸24个采样点采集珠江河口水体,参照卫生部疾病控制司1999年版《霍乱防治手册》进行霍乱弧菌的分离培养和菌型鉴定,用7500a电感耦合等离子体质谱仪进行水样各离子浓度的检测。结果:共采集水样576份,检出51株O1/O139群霍乱弧菌,检出率为8.85%。夏天检出O1/O139群霍乱弧菌数占30%以上。Al、Mn及Ni与O1/O139群霍乱弧菌检出率之间有一定的线性关系。市内、外珠江水中Mg2+、K+、Ca2+、Mn2+、Ni2+、Se4+、Sb3+含量和PH值存在显著或极显著差异。结论:珠江河口水中检出O1/O139群霍乱弧菌与珠江河口水体理化因素Al、Mn及Ni有一定相关性。更多还原

【Abstract】 Objective:To investigate the correlation between Vibro Cholerae O1/O139 detection rate and the physical and chemical factors of Pearl River Estuary water and provide a reference for cholera prevention and control.Methods: 24 sampling points along the Pearl River have been set to collect the Pearl River estuary water per month since 2010.With reference to Cholera Prevention and Control Manual(1999) by the Disease Control Division of the Ministry of Health,Vibrio cholerae was isolated for culture and identification.The ion density of water samples were tested by 7500a inductance coupled plasma mass spectrometry instrument.Results: 576 water samples were collected totally.51 Vibrio choleras O1/O139 strains were detected and the detection rate was 8.85%.The detection rate of Vibrio cholerae O1/O139 in summer accounted for more than 30%.Vibrio cholerae detection rate had linear relationship with Al3+,Mn2+,Ni2+.In the urban district and outside the Pearl River,the significant difference existed between the contents of Mg2+,K+,Ca2+,Mn2+,Ni2+,Se4+,Sb3+ and PH value in water samples.Conclusion: The detection Vibrio cholerae of O1/O139 has some correlation to physical and chemical factors(Al3+,Mn2+.Ni2+)of Pearl River estuary water.更多还原