【摘要】 [目的]研究从自然染病的桃小食心虫幼虫上分离的球孢白僵菌TST05菌株的生物学特性。[方法]测定不同培养基、外界不同温度和湿度对该菌株菌丝营养生长及孢子萌发的影响。[结果]该菌株在PDA、PPDA、SDAY、SMAY 4种培养基上均生长良好,菌落厚而致密,产孢量均大于3.95×107孢子/mL;菌株适应的温度和湿度范围宽,15～30℃之间,RH 30%～100%之间孢子均可萌发、生长和产孢。随着温度接近25℃、湿度增大,孢子的萌发率、菌落的生长速率和产孢量都显著增加。15℃、RH 100%时产孢量为1.32×107孢子/mL;25℃、RH 30%时,产孢量也能达到1.37×107孢子/mL;25℃、RH 100%时产孢量达到6.19×107孢子/mL。15℃、RH 100%孢子萌发率为52.28%;25℃、RH>80%时,孢子萌发率都能达到90%以上。[结论]TST05菌株能适应北方干旱低温条件,可开发成为防治桃小食心虫的生物制剂。更多还原

【Abstract】 [Objective] The biological characteristics of Beauveria bassiana TST05,a strain originally isolated from the naturally infected larvae of Carposina sasakii(Matsumura)(Lepidoptera: Carposinidae),was investigated.[Method] The effects of culture media,outside temperature and humidity on the mycelium growth,spore germination of the strain were studied.[Result] The strain could grow well in the five culture media tested,namely PDA,PPDA,SDAY and SMAY.The colonies were sick and dense,and the spore yield exceeded 3.95×107spores/mL in these culture media.The TST05 strain was adapt to a wide range of temperature and humidity.The spore germination,mycelium growth and sporulation were normal at 15-30 ℃ and RH 30%-100%.With the temperature approaching to 25 ℃ and the humidity increased,the rate of spore germination and mycelium growth and the yield of spore were all visibly increasing.The spore yield was 1.32×107spores/mL under 15 ℃ and RH 100%;1.37×107spores/mL under 25 ℃ and RH 30%,and 6.19×107spores/mL under 25 ℃and RH 100%.The rate of spore germination was 52.28% under 15 ℃ and RH 100%,and over 90% under 25 ℃ and RH>80%.[Conclusion] The strain TST05 was adapt to low temperature and humidity in the north and could become a biological control agent of C.sasakii.更多还原