【摘要】 目的观察牛磺酸对沙土鼠短暂性前脑缺血的保护作用。方法结扎沙土鼠双侧颈动脉建立前脑缺血模型,缺血5 min后进行再灌注。在缺血1 h后经静脉给予2.5 mg/kg、5 mg/kg、15 mg/kg及50 mg/kg牛磺酸。再灌注7 d时,对沙土鼠的神经功能行为缺损进行评分,并留取全脑,观察海马CA1区锥体细胞的死亡情况。结果 2.5 mg/kg牛磺酸虽然改善前脑缺血5 min再灌注7 d时沙土鼠的行为,降低海马CA1区锥体细胞的死亡,但与缺血对照组相比,无统计学意义,5 mg/kg、15 mg/kg及50 mg/kg牛磺酸可明显改善前脑缺血沙土鼠的行为缺损(P分别为0.001、0.006和0.037),降低海马CA1区锥体细胞的死亡(P均<0.001),其中,5 mg/kg、15 mg/kg和50 mg/kg牛磺酸组的锥体细胞的死亡低于2.5 mg/kg牛磺酸组(P分别为0.001、0.001和0.005)。结论牛磺酸能以剂量依赖方式降低沙土鼠前脑缺血损伤,提示牛磺酸可能是一种有效的短暂性缺血的大脑保护剂。更多还原

【Abstract】 Objective To investigate the protection of taurine against transient forebrain ischemia in gerbils. Methods The model of transient forebrain ischemia in gerbil was induced through ligating bilateral carotid arteries for 5 min.The dose of 2.5,5,15 or 50 mg/kg of taurine was administrated intravenously 1 h after ischemia.The behavior and the neuronal death in hippocampal CA1 region were evaluated 7 days after induction of ischemia. Results Treatment with 2.5 mg/kg of taurine improved gerbil behavior and reduced the neuronal death in hippocampal CA1 region,although it did not reach statistical significance.Taurine at dose of 5,15 or 50 mg/kg improved gerbil behavior(P=0.001,0.006,and 0.037,respectively), and reduced the neuronal death in hippocampal CA1 region significantly(all P=0.0001). Moreover,the efficacies of 5,15,and 50 mg/kg of taurine were better than that of 2.5 mg/kg of taurine(P=0.001,0.001,and 0.005,respectively). Conclusions Taurine attenuates the brain damage due to transient forebrain ischemia in a dose-dependent manner,suggesting that taurine is an efficacious neuroprotectant.更多还原