【摘要】 目的了解肿瘤坏死因子α在吸烟2型糖尿病大鼠脂肪组织中的表达,探讨吸烟对2型糖尿病发生发展的作用。方法将60只大鼠分为正常对照组、2型糖尿病非吸烟组及2型糖尿病吸烟组,分别检测空腹血糖及胰岛素水平,PCR法检测肿瘤坏死因子α在脂肪组织中的表达。结果肿瘤坏死因子α在吸烟2型糖尿病组大鼠脂肪组织中的表达明显高于2型糖尿病非吸烟组及正常对照组。2型糖尿病非吸烟组较对照组上调2.7倍,2型糖尿病吸烟组较对照组上调8.1倍,结果均有统计学意义,P<0.05。结论吸烟可能通过增强肿瘤坏死因子α的表达增加胰岛素抵抗,促进2型糖尿病的发生和发展。更多还原

【Abstract】 Objective To understand the expression of tumor necrosis factor-αin fat tissue of smoked type 2 diabetic rats,to discuss the effect of smoking in the occurrence and development of type 2 diabetes millitus.Methods Divided 60 Wister rats into normal control group,type 2 diabetes group and smoked type 2 diabetes group.Levels of fasting blood glucose and insulin were detected respectively.Tumor necrosis factor-α in fat tissue was detected by PCR.Results The expression of tumor necrosis factor-αin smoked type 2 diabetes group was obviously higher than that of type 2 diabetes group and normal control group.2.7 times in type 2 diabetes group than normal control group,8.1 times in smoked type 2 diabetes group than normal control group.Conclusion Smoking could enhance insulin resistance through decreasing the expression of tumor necrosis factor-αand promote the increase and development of diabetes.更多还原