【摘要】 建立了猪肉中巴喹普林药物残留的超高效液相色谱-串联质谱测定方法,样品用乙酸乙酯提取,吹干后用60%乙腈水(0.1%甲酸)溶解,供液相色谱-串联质谱法分析,内标法定量。结果表明:巴喹普林标准溶液在100～800μg/L范围内呈现良好线性r,2均大于0.998;方法检出限为5μg/kg,定量限为10μg/kg;巴喹普林在10～80μg/kg添加范围内的平均回收率为90.8%～108%,批内、批间RSD均小于6.9%。该方法简便快捷、灵敏度高、定性准确,能满足食品安全检测有关法规的要求。更多还原

【Abstract】 An ultral performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) method was established for the determination of baquiloprim residues in pig muscle.The samples were extracted by Ethyl acetate,dissolved by 60% acetonitril(0.1% Formic acid)and determinatied on UPLC-MS/MS After evaporation.The method was quantified by Internalstandard method.The calibration curves were linear between the peak areas and the concentrations of 20~800 μg/L with correlation coefficients(r2)more than 0.998.The limit of detection of baquiloprim was 5 μg/kg,and the limit of quantitation was 10 μg/kg.The average recoveries from spiked muscle at concentrations of 10~80 μg/kg ranged 90.8%~108% and the relative standard deviations of intra-and inter-batch were both less than 6.9%.更多还原