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副猪嗜血杆菌外膜蛋白表型分析

Analysis of outer membrane proteins phenotype of Haemophilus parasuis

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【作者】 朱必凤杨旭夫彭凌韦昭玉

【Author】 ZHU Bi-feng1,YANG Xu-fu1,PENG Ling1,WEI Zhao-yu2(1.Yingdong College of Life Science of Shaoguan University,Shaoguan,Guangdong 512005,China;2.Life Science College of Nanchang University,Nanchang 330047,China)

【机构】 韶关学院英东生命科学学院南昌大学生命科学学院

【摘要】 采用SDS-PAGE测定了82个副猪嗜血杆菌分离株细胞外膜蛋白(OMP),比较不同临床背景分离株的OMP表型差异,分析了OMP与毒力菌株的相关性。结果表明,外膜蛋白分为3种类型:PAGE I、PAGE II和PAGEⅢ型。约34%患病猪分离株属于PAGE I型,只有约9%健康猪分离株属于PAGE I型,说明36 000~40 000蛋白与菌株的毒力相关。

【Abstract】 The out membrane protein(OMP) in 82 isolations of Haemophilus Parasuis were determined by SDS-PAGE.We compared the diversity of the OMP phenotype of isolates from diffent clinical background to explore the associativity between OMP and virulence pathogen.The results showed that isolates are divided into 3 major PAGE type groups as differece of Rf.PAGE I is characterized betwee 36 000 and 40 000 OMP,and included about 34% isolates recovered from disease pigs and 9% isolates recovered from healthy pigs;PAGE II is characterized by absence of the 36 000-40 000 proteins.It is shown that the 36 000-40 000 OMP could be related to virulence.

【基金】 香港霍英东铭源基金(HK314-14591);广东省农业攻关项目(2004B20201019);韶关市科技创新项目(2010140473)
  • 【文献出处】 中国兽医学报 ,Chinese Journal of Veterinary Science , 编辑部邮箱 ,2012年11期
  • 【分类号】S852.612
  • 【被引频次】2
  • 【下载频次】94
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