【摘要】 【目的】探索提高心肌梗死致心力衰竭大鼠模型构建成功率的方法 ,及心肌梗死后2周能否有效形成心力衰竭的问题。【方法】将40只雄性SD大鼠随机分为对照组(不结扎冠脉,n=10)和心衰模型组(n=30)。分别对大鼠实施戊巴比妥钠腹腔注射麻醉、经口腔气管插管、开胸后通过悬吊结扎法准确地结扎冠状动脉左前降支根部。2周后行超声心动图、血流动力学、血浆炎症因子水平和心肌Masson染色及胶原容积分数的测定。【结果】与对照组比较,心衰模型组的EF%(78.05±7.36 vs.45.69±7.54)呈显著的下降,而LVEDP/mmHg(-3±3 vs.9±5)呈显著的升高(P<0.05)。另外,心衰模型组血浆炎症因子水平(IL-1β、IL-6及TNF-α)及心肌间质CVF呈显著的上升(P<0.05)。【结论】心肌梗死后2周即可成功构建心衰模型,通过悬吊结扎法结扎冠状动脉可大大提高心衰模型构建的成功率。更多还原

【Abstract】 【Objective】This study was designed to explore a better method for establishing model of congestive heart failure in rats after myocardial infarction and whether two weeks after coronary artery ligation could establish congestive heart failure.【Methods】Forty male SD rats were randomly divided into two groups:Control group(without coronary artery ligation,n = 10) and heart failure group(n = 30).Rats were anesthetized by intraperitoneal administration of pentobarbital sodium,oral tracheal intubation under direct vision,following left thoracotomy,a large transmural myocardial infarction was induced by suspended the ventricle and then ligated left anterior descending coronary artery at 1 mm from the origin.Echocardiography,hemodynamic measurements,plasma inflammatory cytokines levels,Masson’s trichrome staining and collagen volume fraction were detected two weeks post-surgery.【Results】Compared with the control group,EF/%(78.05 ± 7.36 vs.45.69 ± 7.54) was significantly reduced,but LVEDP/mmHg(-3 ± 3 vs.9 ± 5) was significantly higher in heart failure group(P < 0.05).In addition,the levels of plasma inflammatory cytokines(IL-1β,IL-6,and TNF-α) and CVF were significantly increased(P < 0.05).【Conclusion】Two weeks after myocardial infarction can be successfully constructed the model of heart failure and through the suspended and then ligated methods could improve model success rate obviously.更多还原