【摘要】 【目的】探讨地塞米松对肠缺血再灌注肺损伤的保护作用。【方法】35只雄性昆明鼠随机平均分为5组,每组7只,分别是假手术组(S);模型组(II/R);模型+地塞米松预处理组(II/R+DP);模型+地塞米松缺血时处理组(II/R+DI);模型+地塞米松再灌注即刻处理组(II/R+DR)。每组实验结束之后留取肺标本,以待进行肺组织病理检测、MDA含量和SOD活性检测、TNF-α检测、NO含量测定、iNOS活性测定。【结果】肠缺血再灌注过程显著地造成了急性肺损伤,体现在病理积分显著降低(P<0.05,II/R vs.S),MDA、TNF-α、NO的含量以及SOD和iNOS活性的上升(P<0.05,II/R vs.S),地塞米松预处理能减轻肠缺血再灌注肺损伤(P<0.05,II/R+DP vs.II/R),而地塞米松缺血期和再灌注即刻处理对肺无明显保护作用。【结论】地塞米松预处理能减轻肠缺血再灌注继发性损伤,缺血期和再灌注即刻用药则无保护作用。更多还原

【Abstract】 【Objective】To investigate the protective effect of dexamethasone on lung injury secondary to intestinal ischemia-reperfusion.【Method】Thirty-five male rats weighting 20～24 g were randomized into sham group,sole IIR group(IIR),or IIR and preconditioning with dexamethasone(II/R+DP),or II/R+DI group(respectively treated with dexamethasone after ischemia began),or II/R+DR group(respectively treated with dexamethasone as soon as reperfusion).At the end of experiment,lung tissue was obtained for histologic assessment and for the measurement of the levels of MDA,SOD,TNF-α,NO,and iNOs.【Result】IIR resulted in lung injury evidenced as significant decreases in lung histological scores(P < 0.05,IIR vs.S),accompanied with concomitant increases of the levels of MDA,SOD,TNF-α,NO and the activity of iNOs(P < 0.05,II/R vs.S).Preconditioning with dexamethasone attenuate IIR mediates lung injury(P < 0.05,II/R+DP vs.II/R),while treated with dexamethasone during the ischemia and reperfusion period were unprofitable to lung.【Conclusion】Preconditioning with dexamethasone attenuate IIR mediates lung injury,while treated with dexamethasone during the ischemia and reperfusion period were unprofitable to lung.更多还原