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利用昆虫病原线虫防治草菇双额岩小粪蝇

Using Entomopathogenic Nematodes for Control of Mushroom Fly, Bifronsina bifrons (Stenhammar) (Diptera: Sphaeroceridae)

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【作者】 刘桂清徐洁莲欧剑峰吴华黄鸿

【Author】 LIU Guiqing,XU Jielian,OU Jianfeng,WU Hua,HUANG Hong*(Guangdong Entomological Institute,Guangzhou 510260,China)

【机构】 广东省昆虫研究所

【摘要】 草菇双额岩小粪蝇Bifronsina bifrons是当前草菇生产中的重要害虫。本文测定了5个线虫品系在(30±0.5)℃下对草菇双额岩小粪蝇的致死率和LT50。结果表明,各线虫品系在浓度4000 IJs mL 1下的LT50范围为2.13~4.71 d,其中夜蛾斯氏线虫Steinernema feltiae SN品系对双额岩小粪蝇的毒力最大,LT50为2.13 d,侵染5 d后的致死率达86.67%。菇房施用4×106IJs.m 2S.feltiae SN品系和嗜菌异小杆线虫Heterorhabditisbacteriophora H06品系悬浮液于培养基表面6 d后,对双额岩小粪蝇的防治效果分别达到70.37%和45.68%,SN线虫对双额岩小粪蝇的防效与阿维菌素相当。

【Abstract】 Mushroom fly,Bifronsina bifrons,is an important insect pest in straw mushroom production in South China.Virulence of five strains of Steinernema and Heterorhabditis to the fly was bioassayed at(30±0.5) ℃ in laboratory.Results showed that LT50values of the five strains to the mushroom fly ranged from 2.13 d to 4.71 d.S.feltiae SN strain was a potential biocontrol agent against the mushroom fly with LT50value of 2.13 d and 86.67% of lethality at the concentration of 4000 IJs mL 1at 5 d after treatment.Field trails demonstrated that when nematode suspensions containing 4×106IJs m 2 were sprayed onto the mushroom beds the day before inoculation,control efficacy of S.feltiae SN strain and H.bacteriophora H06 strain against the mushroom fly at 6 d after treatment was 70.37% and 45.68%,respectively,and the control efficacy of S.feltiae SN strain was almost the same as that of avermectins.

【基金】 广东省科技成果转化启动项目(2010B060600008);广州市科技计划项目(2006Z3-E0711)
  • 【文献出处】 中国生物防治学报 ,Chinese Journal of Biological Control , 编辑部邮箱 ,2012年01期
  • 【分类号】S436.462
  • 【被引频次】9
  • 【下载频次】182
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