【摘要】 2-苯乙醇是一种具有玫瑰香气的芳香醇,作为重要香味添加剂之一广泛应用在食品、化妆品及烟草行业中。国内外崇尚利用微生物发酵法生产天然2-苯乙醇。目前国内外报道的单相单菌株产量分别达到3.6g/L和3.8g/L。本文从9株酵母及9株白地霉中筛选出一株2-苯乙醇产量达1.6886g/L的酿酒酵母菌株TYQ和另一株产量达1.0025g/L的白地霉菌株GS10A。经单因素及正交试验对酵母TYQ培养基优化,获得最佳培养基组成为:葡萄糖80g/L,L-苯丙氨酸35g/L,酵母粉10g/L,蛋白胨10g/L。该菌株在接种量10%(v/v)、28℃、120r/min条件下发酵36h的2-苯乙醇产量为4.4025g/L。更多还原

【Abstract】 2-phenylethanol is an aromatic alcohol with a rose-like odor.It is a very important fragrance used in the cosmetic and tobacco industries and is also largely used in food industries.More and more people like 2-phenythanol from microorganism than synthetic from chemicals.In one-phase fermentation,the yield of 2-phenylethanol by single strain of yeasts are 3.6g/L and 3.8g/L in China and abroad respectively.Two strains were selected during the initial screen:a strain of Saccharomyces cerevisiae TYQ which could produce 1.6886g/L 2-phenylethanol and another strain of Geotrichum candidum GS10A which could produce 1.0025g/L 2-phenylethanol.Medium of the TYQ was optimized by the single factor test and orthogonal test.The optimized medium contains glucose 80g/L,L-phenylalanine 35g/L,yeast extract powder 10g/L,peptone 10g/L.Grown under the condition of 10%(v/v)inoculation,28℃ and 120r/min for 36h,the yeast TYQ yielded 2-phenylethanol of 4.4025g/L.更多还原