【摘要】 目的验证HBV表面抗原大蛋白(LHBs)与人胰腺突触角蛋白6(STX6)在细胞内是否存在相互作用,为进一步研究HBV影响糖、脂代谢机制奠定研究基础。方法构建真核表达质粒pACT-STX6,采用哺乳动物双杂交技术验证LHBs与STX6之间的相互作用。结果实验组和各阴性对照组相对荧光素酶活性值均存在差异,且均具有显著统计学意义(P<0.01)。结论 LHBs与人胰腺STX6在胰腺细胞内存在相互作用。更多还原

【Abstract】 Objective To confirm the intra-cellular interaction between HBV large surface protein (LHBs) and homo sapiens syntaxin 6 protein (STX6), which brought some new clues for studying on the molecular biology mechanism of glucose and lipid. Methods Eukaryotic expression vector pACT-STX6 and confirmed the interaction between LHBs and homo sapiens syntaxin protein through mammalian two-hybrid experiment. Results The research constructed eukaryotic expression vector pACT-STX6. Mammalian two-hybrid experiment showed that there were significant differences in the relative luciferase activity value between experimental group and each negative control group were(P< 0.05). Conclusions LHBs might be interacted with homo sapiens syntaxin 6 protein in cells.更多还原