【摘要】 目的:提取威灵仙总皂苷(TSRC),体外作用于急性早幼粒细胞白血病HL60细胞,研究TSRC对HL60细胞的作用机制。方法:将25～400 mg·L-1的TSRC在体外与HL60细胞共同培养,WST-1法检测TSRC对HL60细胞的增殖抑制作用;采用细胞形态学、透射电镜观察细胞、AnnexinV/PI双染色研究TSRC诱导HL60细胞凋亡作用;采用Wright’s-Giemsa染色细胞形态学观察、硝基蓝四氮唑(NBT)还原试验、细胞免疫表型CD11b检测等方法研究TSRC诱导HL60细胞分化作用。结果:50～400 mg·L-1TSRC在体外对HL60细胞增殖有明显抑制作用,呈现剂量和时间的量效关系;不同浓度TSRC作用24 h后均能显著促进HL60细胞凋亡;但具有统计学意义的诱导分化现象不明显。结论:TSRC在体外可有效抑制HL60细胞增殖,并诱导HL60细胞凋亡,但对HL60细胞无明显的诱导分化作用。更多还原

【Abstract】 Objective:To study the effect of total Saponin of Radix Clematidis(TSRC) on HL60 leukemia cell in vitro.Method:In vitro,HL60 cells were incubated with 25-400 mg · L-1TSRC,the proliferation inhibition rate of HL60 was tested by WST-1.Cell morphology,transmission electron microscope(TEM) and Annexin-V/PI were used to investigate the apoptosis of HL60 cells.The differentiations of HL60 was analyzed by morphology of Giemsa stain,nitroblue tetrazdium(NBT) reduced method.The expressions of CD11b and CD33 induced by these drugs were measured by flow cytometry(FCM) at different time.Result: As compared with blank control group,Different concentrations from 50-400 mg · L-1 TSRC could obviously inhibit the growth of HL60 cells with time and dose dependence;While no statistical significance in the differentiation phenomenon was observed.Conclusion: In vitro,HL60 cell’s proliferation inhibition and apoptosis could obviously induced by TSRC.But HL60 cells could not be induced differentiation by TSRC.更多还原