【摘要】 目的:研究芍药甘草汤及其拆方对H2O2诱导损伤大鼠小肠上皮细胞的保护作用。方法:实验设空白对照组,H2O2模型组,白芍(Paeoniae Radix,PR)组,甘草(Licorice,L)组,白芍甘草不同比例组。药物组在药物作用24 h后,与模型组一起加入200μmol.L-1的H2O2作用2 h,空白对照组正常培养,用MTT方法检测细胞活性,Annexin V/PI双染检测细胞凋亡。RT-qPCR检测Caspase-3和Bax mRNA表达。结果:与空白对照组比较,在H2O2损伤后,模型组细胞MTT检测吸光度(A)明显降低,存活细胞明显减少,凋亡增高,Caspase-3表达明显增高,Bax mRNA无明显变化;与H2O2模型组比较,PR 200μg.mL-1,PR 100μg.mL-1,L 100μg.mL-1,L50μg.mL-1,PR 200μg.mL-1/L100μg.mL-1,PR 100μg.mL-1,100μg.mL-1,PR100μg.mL-1,50 mg.L-1组A明显增高,细胞凋亡明显减轻,Caspase-3表达明显降低。结论:白芍,甘草单用,以及白芍与甘草配伍均对H2O2诱导损伤的小肠上皮细胞有保护作用,其机制可能与抑制Caspase-3 mRNA的表达相关。更多还原

【Abstract】 Objective:The present study was designed to observe the effect of Paeornla Licore Tang(PR/L) and its disassembled formula Shaoyao(PR),Ganchao(L) on damage of rat IEC-6 cells induced by H2O2,and also to investigate the potential mechanism.Method: IEC-6 cells were cultured with blank control medium,PR/L,PR,or L of the fixed dosages previously for 24 h,and then stimulated with 200 μmol · L-1 H2O2 for 2 h.Cell survival was measured by MTT assay.Cell apoptosis was evaluated by using flow cytometry with Annexin V / PI staining.Expression of Caspase-3 mRNA was detected by RT-qPCR.Result: Compared with the controls,the H2O2 model showed decrease in survival and expression of Caspase-3 mRNA,and increase in apoptosis.Compared with the H2O2 model,after treatment of PR 200 μg · mL-1 or 100 μg · mL-1,Gancao of 100 μg · mL-1 or 50 μg · mL-1,or both PR and L for 24 h,cell damage and cell apoptosis were reduced significantly,and expression of Caspase-3 mRNA and protein was both increased.Conclusion: Shaoyao,Gancao,and Shaoyao Ganchao Tang can protect IEC-6 cells from damage by H2O2,which may be due to the decrease in Caspase-3 expression.更多还原