【摘要】 目的:探讨补阳还五汤含药血清对缺氧缺糖(oxygen-glucose deprivation,OGD)损伤PC12细胞形态和活力的影响。方法:采用中药血清药理学方法制备补阳还五汤含药血清,以5%,10%,20%的含药血清作用于OGD损伤PC12细胞,于倒置显微镜下观察细胞形态,用四甲基偶氮唑盐法检测细胞活力。结果:PC12细胞OGD损伤后形态发生改变、细胞活力降低;5%,10%补阳还五汤含药血清作用于OGD损伤PC12细胞12,18,24 h,均可减轻细胞形态损伤、提高细胞活力(P<0.01);而20%补阳还五汤含药血清随作用时间的延长对OGD损伤PC12细胞的形态和活力具有双向调节作用。结论:一定浓度的补阳还五汤含药血清对OGD损伤PC12细胞的形态和活力具有保护作用。更多还原

【Abstract】 Objective:To investigate effects of Buyang Huanwu decoction containing serum on the morphology and viability of PC12 cells during oxygen-glucose deprivation.Method:Buyang Huanwu decoction containing serum was obtained by the seropharmacological method.PC12 cells were treated with different concentrations(5%,10%,20%) of the serum during oxygen-glucose deprivation.The cell morphology was observed with inverted microscopy,and the cell viability was examined by methyl thiazolyl tetrazolium assay.Result:The cell morphology was changed and the cell viability was decreased during oxygen-glucose deprivation,and the injured degree of cell morphology was relieved and the cell viability was increased in PC12 cells which treated with concentrations of 5%,10% Buyang Huanwu decoction medicated serum for 12,18,24 h(P<0.01).However,20% Buyang Huanwu decoction containing serum had the bidirectional regulation effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation.Conclusion:The certain concentration of Buyang Huanwu decoction congtaining serum has protective effects on the morphology and viability of PC12 cells during oxygen-glucose deprivation.更多还原