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HPLC测定香青兰中齐墩果酸和熊果酸的含量
Determination of Oleanolic Acid and Ursolic Acid Content in Dracocephalum moldovia by RP-HPLC
【摘要】 目的:采用反相高效液相色谱法测定香青兰中齐墩果酸和熊果酸的含量。方法:采用Kromasil C18柱(4.6 mm×250 mm,5μm),流动相甲醇-0.2%磷酸二氢钠(88∶12),流速1.0 mL·min-1,检测波长210 nm,柱温25℃。结果:齐墩果酸与熊果酸线性范围分别为0.42~2.10μg(r=0.999 4),0.436~2.18μg(r=0.999 7);平均回收率分别为齐墩果酸98.9%,RSD 1.14%(n=6);熊果酸98.1%,RSD 1.80%(n=6)。结论:该方法快速、准确、可靠,可用于同时测定香青兰中齐墩果酸和熊果酸的含量。
【Abstract】 Objective: Use the method of HPLC to determine the conten of oleanolic acid and ursolic acid in Dracocephalum moldovia.Method: The content of oleanolic acid and ursolic acid was detected by HPLC.The separation was carried out on a Kromasil C18 column(4.6 mm×250 mm,5 μm).The mobile phase was methanol-0.2% sodium dihydrogen phosphate(88∶ 12);the flow rate was 1.0 mL·min-1;the detective wavelength was set at 210 nm.The column temperature was at 25 ℃.Result: The linear ranges of oleanolic acid and ursolic acid were 0.42-2.10 μg(r=0.999 4) and 0.436-2.18 μg(r=0.999 7),respectively.The average recoveries were 98.9% with RSD of 1.14%(n=6) and 98.1% with RSD of 1.80%(n=6),respectively.Conclusion: This method was rapid,accurate and reliable;it could be used to determine the content of oleanolic acid and ursolic acid in D.moldovia simultaneously.
【Key words】 Dracocephalum moldovia; oleanolic acid; ursolic acid; HPLC;
- 【文献出处】 中国实验方剂学杂志 ,Chinese Journal of Experimental Traditional Medical Formulae , 编辑部邮箱 ,2012年15期
- 【分类号】R284.1
- 【被引频次】14
- 【下载频次】238