【摘要】 目的:检测湖南衡阳产野菊不同部位(叶、花、花蕾、嫩茎及老茎)中绿原酸和3,5-二咖啡酰奎尼酸的含量。方法:以80%的乙醇为溶剂,用超声法提取野菊不同部位中的绿原酸和3,5-二咖啡酰奎尼酸。采用高效液相色谱法检测绿原酸和3,5-二咖啡酰奎尼酸的含量,以0.05%磷酸水溶液-乙腈为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长327 nm。结果:绿原酸和3,5-二咖啡酰奎尼酸的质量浓度在10～100 mg.L-1(r=0.996,r=0.992)与峰面积呈良好的线性关系(n=5),平均回收率(n=6)分别为98.5%,99.7%。野菊叶、花、花蕾、嫩茎及老茎中绿原酸的含量分别为1.08%,0.44%,0.39%,0.58%及0.52%;3,5-二咖啡酰奎尼酸的含量分别为1.66%,1.01%,0.82%,0.93%,0.64%,其中野菊叶中绿原酸和3,5-二咖啡酰奎尼酸的含量最高。结论:野菊不同部位均含有绿原酸和3,5-二咖啡酰奎尼酸,但它们的含量存在明显差异。更多还原

【Abstract】 Objective:To analyze the contents of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the different parts of Chrysanthemum indicum,which sources from hengyang,Hunan province.Method:Chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the different parts of C.indicum were extracted by the method of ultrasonic method with 80% ethanol and analyzed by RP-HPLC at the wavelength of 327 nm with the flow rate of 1.0 mL·min-1.Meanwhile,0.05% H3PO4 aqueous solution and acetonitrile were used as the mobile phase with gradient elution.Result: The linear range(n=5) of chlorogenic acid(r=0.996)and 3,5-O-dicaffeoylquinic acid(r=0.992) was 10-100 mg·L-1.The average recoveries(n=6) of chlorogenic acid and 3,5-O-dicaffeoylquinic acid were 98.5% and 99.7%,respectively.The contents of chlorogenic acid in the different parts of C.indicum including leaves,flowers,buds,tender stems and old stems was 1.08%,0.44%,0.39%,0.58% and 0.52%,respectively,while the contents of 3,5-O-dicaffeoylquinic acid was 1.66%,1.01%,0.82%,0.93% and 0.64%,respectively.The contents of chlorogenic acid and 3,5-O-dicaffeoylquinic acid in the leaves are the highest of the all tested samples.Conclusion:Different parts of C.indicum tested all have chlorogenic acid and 3,5-O-dicaffeoylquinic acid,but the contents of which was obvious difference.更多还原