【摘要】 目的:对广西产匙羹藤叶中牛弥菜醇A的含量进行测定,为科学评价及有效控制其质量提供依据。方法:采用高效液相色谱法,以氨基柱(4.6 mm×250 mm,5μm)为色谱柱,流动相乙腈-水(87∶13),流速1.0 mL.min-1,检测波长207nm。结果:牛弥菜醇A在2.4～12μg与峰面积成良好的线性关系(r=0.999 7),平均回收率为101.39%,RSD 0.74%。结论:所建立的方法准确,快速,灵敏度高,重复性好,可作为广西产匙羹藤叶的质量控制方法。更多还原

【Abstract】 Objective: To determine the content of conduritol A in the leaf of Gymnema sylvestre.Method: HPLC was adopted,the separation was performed on a Thermo NH2 column(4.6 mm×250 mm,5 μm),acetonitrile-water(87∶ 13) was used as the mobile phase,the flow rate was 1.0 mL·min-1,the detection wavelength was set at 207 nm.Result:The liner range of conduritol A was 2.4-12 μg(r=0.999 7),the average recovery rate was 101.39%,RSD was 0.74%.Conclusion: The method was accurate,sensitive,quick and simple.It can be used as the quality control method of the leaf of G.sylvestre.更多还原