【摘要】 目的:研究牛蒡苷胶囊对阿霉素肾病大鼠的保护作用。方法:采用一次性尾静脉注射(阿霉素ADR)复制微小病变型肾病模型,。SD雄性大鼠随机分为空白组、模型组、阳性药醋酸泼尼松片5 mg.kg-1组、牛蒡苷胶囊高、中、低剂量组(767,383,192 mg.kg-1),ig给药6周。于每周末将大鼠放入代谢笼中,收集24 h尿液,用全自动生化仪测定尿蛋白含量。于末次给药后24 h,眼眶静脉丛采血,于全自动生化仪测定白蛋白(ALB)、甘油三酯(TG)、胆固醇(TC)含量;采用酶联免疫法测定血清转化生长因子-β1(TGF-β1)水平;采用黄嘌呤氧化酶法和硫代巴比妥法分别测定血清超氧化物歧化酶(SOD)和丙二醛(MDA);肾组织病理学检测。结果:与模型组比较,牛蒡苷胶囊高、中剂量能减少阿霉素肾病大鼠的尿蛋白含量,提高血清ALB含量;牛蒡苷胶囊3个剂量组能降低血清TG和TGF-β1含量;牛蒡苷高剂量(767 mg.kg-1)能提高血清SOD活性、降低血清MDA含量;牛蒡苷胶囊3个剂量组能够促进肾组织病理改变的修复。结论:牛蒡苷胶囊对阿霉素肾病大鼠的保护作用可能与其清除氧自由基、抑制脂质过氧化反应、抑制肾小球纤维化等作用有关。更多还原

【Abstract】 Objective:To study the protective effect of arctinin capsule on the nephritic rat induced by adriamycin(ADR).Method: Minimal change nephrotic rat model was induced by intravenous injection of ADR,male SD rat were randomly divided into 6 groups:Blank group,Model group,positive group,arctinin capsule high-dose group(767 mg·kg-1),middle-dose group(383 mg·kg-1)and low-dose group(192 mg·kg-1),treated for 6 weeks.Result: Campared with the rats in model group,arctinin capsule high-dose,middle-dose could reduced the proteinuria of the nephritic rat induced by adriamycin,increase the level of serum albumin(ALB);Arctinin capsule all the 3 doses could cut down the level of serum triglyeride(TG)and transforming growth factor-β1(TGF-β1);Arctinin capsule high-dose(767 mg·kg-1) could increase the level of serum superoxide dismutase(SOD)and cut down the level of serum malondialdehyde(MDA);at the same time,arctinin capsule all the 3 doses could significanty ameliorated the change of pathology in renal.Conclusion:The protective action of arctinin capsule on the nephritic rat induced by adriamycin may be related to the purification on free-radical,the inhibition of lipid peroxidation and glomerular fibrosis.更多还原