【摘要】 目的:观察当归芍药散(DSS)对垂体后叶素(Pit)所致小鼠心肌缺血的影响。方法:昆明种小鼠100只,分为空白对照组、模型组、DSS低剂量组(生药30 g.kg-1.d-1)、DSS高剂量组(生药60 g.kg-1.d-1)和阳性对照组(复方丹参滴丸0.174 g.kg-1.d-1),ig连续5 d,末次药后1 h,ip Pit(30 U.kg-1)进行造模。记录ip Pit前10 min和注射后5,15,25 min时点Ⅱ导联心电图;检测血清一氧化氮(NO)水平、内皮型一氧化氮合酶(eNOS)活性(免疫组化法)以及心肌组织匀浆超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:DSS高、低剂量组与模型组相比,心率均明显改善,具有显著差异(P<0.05或P<0.01);DSS高、低剂量血清NO水平分别为(79.35±25.18),(26.46±18.24)μmol.L-1,明显高于模型组(13.66±3.83)μmol.L-1(均P<0.01)。DSS高、低剂量eNOS活性为(0.259±0.037),(0.241±0.029),均强于模型组(0.176±0.036),P<0.01)。DSS高、低剂量心肌SOD活性分别为(269.55±20.65),(263.58±21.04)U.mg-1,明显高于模型组(222.75±23.44)U.mg-1(P<0.01);DSS高、低剂量心肌MDA含量分别为(3.58±1.01),(4.22±0.75)nmol.mg-1,明显低于模型组(5.25±1.42)nmol.mg-1(P<0.05或P<0.01)。结论:当归芍药散能有效防治由Pit所引起的心肌缺血及缺血损伤,具有保护心肌细胞的作用。更多还原

【Abstract】 Objective:To observe the effects of Danggui Shaoyao San(DSS) on experimental myocardial ischemia in mice.Method:One hundred healthy male Kunming mice were divided into five groups at random:blank control group,model group,DSS low-dose group(raw herb 30 g·kg-1·d-1),DSS high-dose group(60 g·kg-1·d-1) and positive control group(Fufang Danshen Diwan 0.174 g·kg-1·d-1).Each group was fed with according medicine from the first day to the fifth day.On the fifth day injections of Pit(30 U·kg-1) was given to every group intraperitoneally except the blank control group to which injections of saline were given intraperitoneally.The myocardial ischemia model was duplicated by pit.The electrocardiogram at the tenth minute before the Pit injection and the 5,15,25 min after the Pit injection was recorded.Thirty minutes after the Pit injections the mice were sacrificed,the blood was obtained and the contents of NO were checked up.The tip of myocardium was made into pathological specimen to observe the activities of eNOS by immunohistochemitry method.The rest myocardium was made into tissue homogenate with saline for checking up the activity of SOD and the content of MDA.Result:Compared with the model group,the heart rates in the DSS high-dose and the low-dose groups were recovered significantly(P<0.01 or P<0.05).The contents of serum NO in the DSS high-dose group,the low-dose group were increased significantly(P<0.01).The activities of eNOS in the DSS high-dose group and the low-dose group were enhanced.The activities of SOD in the DSS high-dose group and the low-dose group were increased significantly(P<0.01),while the contents of MDA were decreased significantly(P<0.05 or P<0.01).Conclusion:The classical prescription DSS could prevent and treat myocardial ischemia injure effectively,protect the myocardial cell.更多还原