【摘要】 目的:对柴胡等7种中草药的超临界CO2萃取物进行抗惊厥作用的比较,并初步探讨其作用机制。方法:将昆明种小鼠1 060只随机分为11组,柴胡CO2萃取物组(柴Ⅰ)、柴胡CO2乙醇提取物组(柴Ⅱ)、石菖蒲CO2萃取物组(石Ⅰ)、石菖蒲CO2乙醇提取物组(石Ⅱ)、天南星CO2乙醇提取物组(天Ⅱ)、钩藤CO2乙醇提取物组(钩Ⅱ)、半夏CO2乙醇提取物组(半Ⅱ)、瑞香狼毒CO2萃取物组(狼Ⅰ)和柏子仁CO2萃取物组(柏Ⅰ)、托吡酯组、生理盐水组,每组分为4～6个剂量组,每个剂量组20只,ig给药,观察各剂量组对最大电休克(maximal electroshock seizure,MES)模型的对抗作用;以上述各药抗MES惊厥的ED50为给药剂量,观察ig各药对小鼠戊四唑惊厥模型(metrazol seizure test,MET)的对抗作用;由小鼠抗MES惊厥的ED95折算得到大鼠ig剂量,建立大鼠皮层定位注射青霉素点燃模型,比较各药对惊厥行为和脑电图的影响。结果:①9种受试中药提取物中天Ⅱ、钩Ⅱ、石Ⅰ、半Ⅱ、狼Ⅰ、柴Ⅰ、石Ⅱ对MES模型均有对抗作用,且量效均呈正相关性。②7种受试中药提取物均可延长MET惊厥潜伏期,数值由大到小排列依次为天Ⅱ、钩Ⅱ、石Ⅰ、半Ⅱ、狼Ⅰ、柴Ⅰ、石Ⅱ,其中天Ⅱ(11.06±4.32)min的作用效果优于托吡酯(9.57±4.47)min。③CO2萃取物较CO2乙醇萃取物起效快,作用持续时间短。(4)半Ⅱ、柴Ⅰ、天Ⅱ、钩Ⅱ、石Ⅰ、石Ⅱ均可不同程度的延长青霉素诱发的痫性发作潜伏期、减轻发作程度,延长痫性放电的潜伏期,减少痫波发放频率,减小放电最高波幅。结论:天Ⅱ、钩Ⅱ、石Ⅰ、半Ⅱ、狼Ⅰ、柴Ⅰ、石Ⅱ均可对抗小鼠MES和MET惊厥;半Ⅱ、柴Ⅰ、天Ⅱ、钩Ⅱ、石Ⅰ、石Ⅱ可抑制大鼠皮层定位注射青霉素诱发的癫痫发作和痫性放电,其中半Ⅱ是对抗青霉素点燃惊厥发作最有效的药物,且对该模型的对抗作用优于托吡酯。更多还原

【Abstract】 Objective:To compare the anticonvulsion effect of supercritical-CO2 fluid extractions(SFE-CO2) of Bupleuri Radix,and explore their mechanism.Method:One thousand and sixty mice were randomly divided into 11 groups:RBⅠgroup,RBⅡgroup,RATⅠgroup,RATⅡgroup,ARⅡgroup,UNⅡgroup,PRⅡgroup,SCLⅠgroup,PSⅠgroup,topamax group and NS group.Every group was divided into 4-6 dose groups.The maximal electroshock seizure(MES) was used to observe the anticonvulsion effect.ED50 dose against MES was considered as the administrated dosage to observe the anticonvulsant affects in metrazol seizure test(MET).The rat seizure model was reduced by cortex localion injection of penicillin to investigate the effect of extractions on the convulsant behaviors and hippocampus EEG(the doses were converted by ED95 of MES).Result:①Except for RBⅡand PSⅠ,all the other seven extractions(PRⅡ,UNⅡ,RATⅠ,PRⅡ,SCLⅠ,RBⅠ,RATⅡ) could dose-dependently antagonize MES in mice.②All of the seven extractions could prolong the latent period of MET in mice,and the sequence from maximum to minimum was RPⅡ,UNⅡ,RATⅠ,PRⅡ,SCLⅠ,RBⅠ,RATⅡ.The effect of RPⅡ(11.06±4.32)min was better than topamax(9.57±4.47)min.③ The extractions with SFE-CO2 showed a faster onset and shorter duration of he anticonvulsant action than that of supercritical-CO2 alcohol fluid extractions(SAFE-CO2).④PRⅡ,ARⅡ,UNⅡ,RATⅠ,RATⅡ and RBⅠ could prolong the latent period of epileptiform discharge,reduce the frequency of epileptiform discharge,and decrease the highest wave of hippocampus EEG.Conclusion:ARⅡ,UNⅡ,RATⅠ,PTⅡ,SCLⅠ,RBⅠ,RATⅡ could dose-dependently antagonize MES and MET in mice;PRⅡ,ARⅡ,UNⅡ,RATⅠ and RBⅠ could antagonize penicillin-kindling seizure in rats.PTⅡ may be the most effective extraction in antagonizing the penicillin-induced seizure,and the effect of which may be better than topamax.更多还原