【摘要】 目的:研究乙醇注入法制备莪术醇脂质体的最佳处方及制备工艺。方法:采用乙醇注入法制备莪术醇脂质体;以包封率和稳定性参数为考察指标,采用正交设计优化处方与制备工艺,高效液相色谱法测定脂质体包封率。结果:最优处方为磷脂70 g.L-1,磷脂与胆固醇质量比8∶1,磷酸盐缓冲液pH 6.0,摩尔浓度0.10 mol.L-1;最佳制备工艺为探头超声时间4min,磁力搅拌速度30 r.min-1,水浴温度50℃,按最佳处方及工艺制得的莪术醇脂质体均匀圆整,包封率为62.91%,平均粒径124 nm,平均Zeta电位-26.7 mV。结论:乙醇注入法制备工艺简单,易于掌握,优选得到的脂质体处方和制备工艺稳定可行。更多还原

【Abstract】 Objective:To study on optimum formulation and preparation technology of curcumol liposome by ethanol injection method.Method:curcumol liposome were prepared by ethanol injection method;taking entrapment efficiency and stability parameter as indexes,formulation and preparation technology were optimized by orthogonal test;entrapment efficiency of liposome was determined by HPLC.Result:Optimum formulation was:phospholipids 70 g·L-1,phospholipids-cholesterol 8∶1,pH of phosphate buffer 6.0,concentration of phosphate 0.10 mol·L-1;Optimum extraction technology was:probe ultrasonic time 4 min,magnetic stirring speed 30 r·min-1,water bath temperature 50 ℃,appearance of prepared curcumol liposome was uniform round,with entrapment efficiency was 62.91%,average particle size was 124 nm and average zeta-potential was-26.7 mV.Conclusion:preparation process of ethanol injection was simple and easy to control.optimized formulation and preparation of curcumol lipsome was stable and feasible.更多还原