【摘要】 目的了解粤北地区并殖吸虫流行分布现状。方法采集并解剖每处调查点山溪中螺蛳及溪蟹,查找并殖吸虫尾蚴和囊蚴。以所获囊蚴人工感染猫、犬,解剖猫、犬查找并殖吸虫成虫。取成虫样本,与GenBank里并殖吸虫的COI基因与ITS2基因序列比对,进行基因序列分析。结果里东、叟里元、下洞河、太坪和小坑5处调查点溪蟹三平正并殖吸虫囊蚴感染率分别为74.54﹪(41/53)、68.91﹪(32/47)、77.77﹪(24/32)、76.92﹪(40/52)和81.5%(22/27),溪蟹物种均鉴定为平和华溪蟹。与GenBank三平正并殖吸虫基因序列比对,5处成虫样本COI基因同源性分别为99.5%、100%、99.5%、99.5%和100%,ITS2基因同源性为100%。大洞调查点的螺蛳检出并殖吸虫短尾尾蚴,感染率为0.058%(1/1,700),螺蛳物种鉴定为放逸短沟蜷,溪蟹检出卫氏并殖吸虫囊蚴,感染率为38.09%(32/84),溪蟹物种亦鉴定为平和华溪蟹。大洞成虫样本COI基因与卫氏并殖吸虫同源性100%,ITS2基因与卫氏并殖吸虫同源性99.5%。结论粤北地区新发现三平正并殖吸虫高度疫源地5处,第二中间宿主为平和华溪蟹。5处疫源地虫种间无差异。卫氏并殖吸虫高度疫源地1处,第一中间宿主为放逸短沟蜷,第二中间宿主为平和华溪蟹。更多还原

【Abstract】 The objective is to determine the epidemiological distribution of Paragonimus in northern Guangdong Province.Cercariae and metacercariae of Paragonimus were isolated from freshwater snails and crabs,and randomly collected at each study sites in northern Guangdong Province.Adult worms were obtained from experimentally infected cats and dogs.The gene sequences of cytochrome c oxidase subunit 1(COI)and second internal transcribed spacer(ITS2)region from adult worms were aligned with those of Paragonimus spp.deposited in GenBank database.Infection rates for the metacercariae of Euparagonimus cenocopiosus in Sinopotamon pinheense recorded at five of the six sites studied were as follows:74.54%(41/ 53)at Lidong,68.91%(32/47)at Souliyuan,77.77%(24/32)at Xiadonghe,76.92%(40/52)at Daping,and 81.5%(22/ 27)at Xiaokeng,respectively.The COI gene sequences of adult worms derived from cats and dogs infected with metacercariae from the above five sites shared 99.5%,100%,99.5%,99.5%,and 100% homology with those fromEuparagonimus cenocopiosus in GenBank,and the ITS2gene was 100%identical.At Dadong,however,38.09%(32/84)of Sinopotamon pinheense were infected with the metacercariae of Paragonimus westermani,and 0.058%(1/1 700)of snails(Semisulcospira Libertina)was infected with cercariae of Paragonimus westermani.The COI gene sequence of adult worms from Dadong was 100% identical to that of Paragonimus westermani in the GenBank,and the ITS2gene sequence was 99.5%identical.Five new high endemic areas of Euparagonimus cenocopiosus,with Sinopotamon pinheense as second intermediate host,and one new high endemic area of Paragonimus westermani,utilizing Semisulcospira Libertina and Sinopotamon pinheense as first and second intermediate hosts,were discovered in northern Guangdong.更多还原