【摘要】 目的应用聚合酶链反应(PCR)与实时taqMan荧光定量PCR(RT-PCR)检测咽拭子标本中的肺炎支原体DNA(Mp-DNA),比较2种方法检测结果的临床诊断价值。方法随机选取临床儿科门诊患儿566例,包括临床治诊Mp感染患儿106例和临床疑似Mp感染患儿460例,分别采用PCR法和RT-PCR法检测,以临床治诊Mp作为参照标准,采用χ2检验评定2种检测方法诊断的灵敏度和特异度,比较2种检测方法对Mp的诊断价值。结果 566份受检患儿的咽拭子标本中,PCR法检测阳性45例(7.95%)(临床治诊Mp感染患儿5例,临床疑似Mp感染患儿40例),RT-PCR法检测阳性175例(30.92%)(临床治诊Mp感染患儿95例,临床疑似Mp感染患儿80例)。RT-PCR法检测咽拭子Mp-DNA诊断Mp感染的敏感度显著高于PCR法(敏感度RT-PCR 89.62%,PCR 4.72%,χ2=146.322,P<0.05),特异度与PCR法的差异无统计学意义(特异度RT-PCR 82.60%,PCR 91.30%,χ2=3.331,P>0.05)。结论应用RT-PCR法检测咽拭子中的Mp-DNA对儿童Mp感染的诊断价值优于应用PCR法检测咽拭子中的Mp-DNA,其中应用RT-PCR检测对Mp感染患儿的早期诊断有更大的临床价值。更多还原

【Abstract】 To compare the clinical diagnostic value between PCR and RT-PCR for M.pneumoniae in swab,we performed both PCR and RT-PCR assays analysis for M.pneumoniae DNA on a total of 566 samples of throat swab from 106 pediatric children with M.pneumoniae and in whom M.pneumoniae was suspected.Among the 566 pediatric children,there were 45(7.95%) PCR-positive specimens and 175(30.92%) RT-PCR-positive specimens.In the 106 pediatric children with M.pneumoniae,5 were positive for PCR,and 95 were positive for RT-PCR.In the 460 pediatric children with symptom of M.pneumoniae,40 were positive for PCR,and 80 were positive for RT-PCR.The sensitivy of Rt-PCR for M.pneumoniae detection appeared to be better than that of PCR.(sensitivity : RT-PCR 89.62 %,PCR 4.72 %,χ2=146.322,P=0.000),but there was no significant difference in the specificity between RT-PCR and PCR(specifitivity:RT-PCR 82.60 %,PCR 91.30%,χ2=3.331,P=0.068).It is concluded that the TaqMan based RT-PCR assay is a rapid,sensitive and specific method for the detection of M.pneumoniae in throat swabs of children in early period of diagnosis.更多还原