【摘要】 目的研究减毒A/California/07/2009ca流感病毒及A/California/07/2009灭活流感病毒之间的体液免疫及细胞免疫效果的差异。方法蔗糖密度梯度离心纯化减毒A/California/07/2009ca流感病毒经滴鼻途径免疫BALB/c小鼠,以灭活病毒肌肉注射组为对照。应用ELISA的方法检测小鼠血清中IgG1、IgG2a及脾淋巴细胞上清液中的IL-6、IL-4和IFN-γ的效价。结果通过对小鼠血清中IgG1、IgG2a及脾淋巴细胞上清液中的IL-6、IL-4及IFN-γ细胞因子的检测,结果显示,灭活流感病毒的IgG1/IgG2a高于减毒流感病毒,同时灭活病毒产生的IL-6和IL-4高于减毒病毒,但IFN-γ低于减毒病毒。结论 A/California/07/2009灭活病毒能够刺激小鼠机体产生较好的体液免疫,但细胞免疫较差,而减毒A/California/07/2009ca流感病毒能够较好激发小鼠体内的体液免疫和细胞免疫反应。通过这些数据我们可以初步断定,A/California/07/2009ca减毒病毒具有较全面的免疫效果,这为流感喷鼻疫苗的研制提供了理论数据参考。更多还原

【Abstract】 The aim of the present study was to observed the differences in effectiveness on humoral and cellular immunity of attenuated swine influenza virus A/California/07/2009ca and inactivated swine influenza virus A/California/07/2009.Attenuated influenza virus(A/California/07/2009ca) were purified by sucrose density gradient,and then intranasally administered in mice,comparing with intramuscular injection of inactivated influenza virus as control.The IgG1 and IgG2a in serum were detected and IL-6,IL-4 and IFN-γ in spleen cells were tested by ELISA.Results demonstrated that IgG1 and IgG2a in serum and IL-6,IL-4 and IFN-γ in spleen cells were all detected.The ratio of IgG1/IgG2a in inactivated influenza virus was higher than that in attenuated influenza virus.And the levels of IL-6 and IL-4 in inactivated influenza virus were higher than those in attenuated influenza virus,while the IFN-γ level in inactivated influenza virus was lower than that in attenuated influenza virus.It’s suggested that inactivated influenza virus(A/California/07/2009) could induce better humoral immunity while poor cellular immunity in mice.However,attenuated influenza virus(California/07/2009ca) could activate both humoral and cellular immune responses.These data were predicated that attenuated influenza virus(A/California/07/2009ca) has a comprehensive immunization,and is important for further studies in term of influenza intranasal vaccine.更多还原