【摘要】 目的:分析张家口地区汉族人乙型肝炎病毒基因分型与耐药变异。方法:分别采用巢式荧光实时定量PCR及荧光实时定量PCR检测张家口地区98例汉族乙肝病毒感染患者基因型及耐药变异,所有患者接受多拉米定抗病毒治疗。结果:张家口地区98例汉族人乙型肝炎病毒基因型主要以B型,C型,B+C混合型为主,其中B型29例(29.59%),C型30例(30.61%),B+C混合型39例(39.80%);发生YMDD变异总变异率为58.16%(57/98)。在发生YMDD变异者中,YVDD变异占61.40%(35/57),YIDD变异占38.60%(22/57)。基因型为B型者YMDD变异率为21.43%(21/98),C型者为33.67%(33/98),B+C混合型者为44.90%(44/98),三者之间差异有统计学意义(P<0.05)。结论:张家口地区汉族人乙型肝炎病毒基因型主要为B型,C型与B+C混合型;B+C混合型患者使用拉米夫定后发生YMDD变异率最高。更多还原

【Abstract】 Objective: To analyze Hepatitis B virus(HBV) genotypes and drug resistance mutations among people in Zhangjiakou. Methods: The genotypes and drug resistant mutation of HBV in 98 infected cases of Han nationality in Zhangjiakou were detected by nested quantitative real-time PCR and real-time fluorescent PCR. Results: The main genotypes among the 98 cases in Zhangjiakou were genotype B(29.59%),C(30.61%) and B+C(39.80%).The total mutation rate of YMDD was 58.16%(57/98),among which,YVDD mutation accounted for 61.40%(35/57),and YIDD mutation was 38.60%(22/57).The YMDD mutation rate in genotype B was 21.43%(21/98),C was 33.67%(33/98) and B+C was 44.90%(44/98) respectively,and the difference among them was statistically significant(P<0.05). Conclusion: The main HBV genotypes of Han nationality in Zhangjiakou are genotypes B,C and B+C,and the genotype B+C has the highest YMDD mutation rate after Lamivudine treatment.更多还原