【摘要】 目的:探讨RSK2在肝细胞癌(HCC)中的表达与化疗药物耐药的关系。方法:将HepG2细胞分为RSK2干扰组(PGCsi3.0-RSK2 siRNA转染细胞),5-FU处理组(10μg/mL 5-FU处理细胞24 h),5-FU处理+RSK2干扰组及空白对照组,采用MTT方法检测各组细胞增殖情况;用Western blot方法检测5-FU处理组和5-FU处理+RSK2干扰组细胞RSK2,c-Jun,Bcl-2,LKB1蛋白表达。结果:与空白对照组比较,沉默RSK2蛋白与5-FU干预均可明显抑制HepG2细胞增殖(均P<0.05),且其联合作用大于两者单独的作用(均P<0.05);与RSK2干扰组比较,5-FU处理+RSK2干扰组细胞RSK2,c-Jun,Bcl-2表达上调,LKB1表达下调(均P<0.05)。结论:抑制RSK2表达可以增强肝癌细胞对化疗药物5-FU的敏感性,机制作用可能与其调节c-Jun,Bcl-2,LKB1蛋白的表达有关。更多还原

【Abstract】 Objective: To investigate the relationship between the expression of ribosomal S6 kinase 2(RSK2) and chemoresistance in hepatocellular carcinoma cells(HCC). Methods: Human HCC HepG2 cells were divided into RSK2 interference group(cells were transfected with PGCsi3.0-RSK2 siRNA plasmid),5-FU treatment group(cells were treated with 5-FU for 24 h),5-FU treatment+RSK2 interference group and blank control group.The cell proliferation of each group was determined by MTT assay and the protein expression levels of RSK2,c-Jun,Bcl-2 and LKB1 were detected by Western blot analysis. Results: Compared with blank control group,the RSK2 interference and 5-FU treatment group both significantly inhibited the proliferation of HepG2 cells(both P<0.05).Moreover,the combined effect of RSK2 interference and 5-FU treatment was greater than each alone(both P<0.05).Compared with RSK2 interference group,the protein expression levels of RSK2,c-Jun and Bcl-2 were increased and LKB1 protein level was decreased in cells of 5-FU treatment+RSK2 interference group(all P<0.05). Conclusion: Inhibition of RSK2 expression can enhance the sensitivity of HCC to chemotherapeutic agent 5-FU,and the mechanism may be related to its regulating the expression of c-Jun,Bcl-2 and LKB1.更多还原