【摘要】 目的制备肿瘤相关糖蛋白-72抗原(TAG-72)嵌合锚定T细胞,通过体外实验检测其对乳腺癌细胞的抑癌活性。方法分离健康人外周血单个核细胞(PBMC),采用脂质体lipofectamineTM2000介导的细胞瞬时转染方法,将已制备的重组真核表达载体即抗TAG-72-scFv-CD3ζ-pcDNA 3.0导入PBMC,以获得TAG-72嵌合锚定T细胞,以此为转染组;用转染空载体pcDNA 3.0的PBMC作为对照组。将转染组嵌合锚定T细胞和对照组PBMC细胞分别与乳腺癌细胞系MCF-7及Bcap37共培养,观察两者对乳腺癌细胞G1期的阻滞率。结果转染组对MCF-7细胞系的G1期阻滞率为(82.3±6.9)%,对照组为(43.4±3.9)%,2组间比较差异具有统计学意义(P<0.05);转染组对Bcap37细胞系的G1期阻滞率为(51.3±4.7)%,对照组为(45.6±2.5)%,2组间比较差异无统计学意义(P>0.05)。结论 TAG-72嵌合锚定T细胞可特异性地抑制TAG-72+乳腺癌细胞的增殖,此将为乳腺癌的临床诊治提供一种有希望的途径。更多还原

【Abstract】 Objective To investigate the effect of tumor associated glycoprotein-72(TAG-72) redirected T lymphocytes on breast cancer cells.Methods Peripheral blood mononuclear cells(PBMCs)were isolated from healthy volunteers.The recombinant vector anti-TAG-72-scFv-CD3ζ-pcDNA 3.0 were transfected into PBMCs by lipofectamineTM 2000(transfection group),PBMCs transfected with plasmid pcDNA 3.0 as control group.MCF-7 and Bcap37 cells were cocultivated with PBMCs of transfection group and control group,respectively,and antitumor response of G1 block was observed.Results G1 block rate of MCF-7 cells in transfection group was(82.3±6.9)%,which was significantly higher than that in control group((43.4±3.9)%,P<0.05).G1 block rate of Bcap37 cells in transfection group was(51.3±4.7)%,and not differed from that in control group((45.6±2.5)%,P>0.05).Conclusion TAG-72 redirected T lymphocytes can inhibit the cell proliferation of TAG-72 positive breast cancer cells,and it may provide valuable tools for the cellular immunotherapy.更多还原