【摘要】 DNA标记技术可从基因组水平上鉴定F1。用SSR标记鉴定水稻突变体杂交F1,为F2群体构建和突变体基因定位提供依据。以经EMS诱导获得的R30白化突变和‘泸恢17’窄叶突变分别作为亲本,组配2个杂交组合,用3个SSR标记对其单株进行鉴定。RM6105检测了R30白化突变ב泸恢17’组合4个单株(1～4号),其中4号单株表现父母本互补的带型,为真实F1,另外3个的标记型与母本相同,为母本自交结实,RM6965和RM5349的鉴定结果与RM6105一致;该3个标记从R30ב泸恢17’窄叶突变组合14个F1单株中(5～18号)检测到3个真实F1(9、12和15号);RM6965、RM5349和RM6105均可对R30与‘泸恢17’组合F1进行鉴定,且结果一致;分子标记对F1进行鉴定,可用于杂交育种。更多还原

【Abstract】 F 1 could be identified by DNA marker technology in genome.To provide the basis for F 2 group construction and gene location of mutants,F 1 hybridized with rice mutants were identified by SSR markers.Mutants of albefaction from R30 and of narrow leaf from ’ Luhui 17 ’ induced by EMS were used as parents respectively in two cross-combinations.F 1 plants from the two combinations were respectively identified by three SSR markers.The four F 1 plants(code 1-4) from mutation of albefaction from R30× ’ Luhui 17 ’ were identified by RM6105.The code 4 showed complementary marker type of both parents.This indicated it was real F 1.The marker types of other three were the same as its female parent.They were self-pollinated for female parent.The results of identification by RM6965 and RM5349 were consistent with that by RM6105.Three(code 9,12 and 15) from the fourteen F 1(code 5-18) from R30×Mutation of narrow leaf from ’ Luhui 17 ’ were identified to be real F 1.F 1 crossed with R30 and ’ Luhui 17 ’ could be identified respectively by the three markers of RM6965,RM5349 and RM6105 and the results were identical.F 1 identification with molecular marker could be applied in cross-breeding.更多还原