【摘要】 为了阐明1999年从转基因后代遗传群体中发现的1株雄性不育植株不育基因的遗传规律及其与现有不育基因的等位性,采用表型观察测量。以及经典的自交和测交手段,研究了该不育材料败育性状的遗传规律。花器官形态特征调查表明:不育株花柱长和花柱外露长度均明显高于同质系的正常可育株,而每朵花的子房直径及花药数量没有明显差异。遗传分析表明:杂合体可育株自交,后代不育株与可育株呈1:3分离,不育株与杂合姊妹可育株测交,不育株与可育株呈1:1分离,表明该核不育材料受隐性单位点控制;与阆A(msc1)、洞A(msc3)等育性位点杂合可育株分别杂交,其F1代单株育性均得到恢复。由其F1代产生的F1:2家系中均出现不育株与可育株呈1:3和7:9两个育性分离群体,表明该材料败育基因为不同于阆A、洞A的不育基因位点。更多还原

【Abstract】 In order to clarify the genetic law of cotton genic male sterile line discovered from the offspring population of a genetically modified cotton population in 1999,phenotype observation,classical self hybridyzation and sib-cross means were applied to analyze the flower phenotypic characteristics,genetic model,and the allelism for Yu 98-8A line.Floral morphology investigation showed that,the length of style and exposed style of sterile plants were significantly higher than the normal of fertile plants,while the diameter of ovary and the number of anthers of each flower had no significant difference.Pollen fertility examination results showed that,there was no pollen within the anther of sterile plants.Genetic analysis of hybrid offspring population of sterile and heterozygous fertile plants and the self-cross of fertile heterozygous plants showed that,the male sterility was controlled by a single recessive gene.The progenies of F 1 from Yu98-8A crossed with Lang A(msc1) and Dong A(msc3) were complete fertile.Several of the F 2 populations derived from the F 1 plants of Yu98-8A crossed with Lang A or Dong A showed fertility segregating with the ratio of sterile plants to fertile plants of 1:3,and the rest F 2 populations showed 7:9.Those results suggested that Yu98-8A male sterile gene was different from that of Lang A and Dong A.更多还原