【摘要】 采用Northern blotting和Western blotting方法分析了聚乙二醇(Polyethylene glycol,PEG)和环己酰亚胺(Cycloheximide,CHX)处理对花生(Arachis hypogaea L.)叶片AhNCED1基因及蛋白表达变化的影响进行了研究,并运用酶联免疫吸附试验(ELISA)对不同胁迫处理后花生叶片内源脱落酸(Abscisic acid,ABA)的含量进行了测定.结果表明,质量分数30%的PEG处理引起四叶期花生叶片AhNCED1基因和蛋白表达量不断上升,分别在处理后24 h和8 h达到最大值,叶片内源ABA含量不断升高.经0.2 mmol/L CHX处理后,AhNCED1基因和蛋白表达水平降低,胁迫后期仍有微弱蛋白表达,叶片内源ABA含量降低,但胁迫处理48 h其内源ABA含量比对照提高了173.7%.表明水分胁迫可诱导新的AhNCED1蛋白表达,引起ABA合成水平升高.更多还原

【Abstract】 The expression of AhNCED1 gene and protein in leaves of peanut in response to Polyethylene glycol(PEG) and Cycloheximide(CHX) treatment were analyzed by Northern and Western blotting,and the abscisic acid(ABA)content in leaves of peanut under different treatment were determined by ELISA.The results showed that the expression of AhNCED1 gene and protein in leaves of peanut were increased along with the water stress time,and that the ABA content was also increased under the water stress.After the CHX treatment,the expression of AhNCED1 gene and protein in leaves of peanut were decreased,and the AhNCED1 protein were still expressed at the latter stress period.The ABA content was also decreased under the CHX stress,but the ABA content was increased by 173.7% when compared to that of the control at 48 hours under the CHX treatment.These above results showed that water stress could induce new AhNCED1 protein synthesis,then increase the content of ABA in peanut leaves.更多还原