【摘要】 为了进行抗癌药物紫杉醇产生菌株的快速筛选,建立了紫杉醇检测的薄层层析(TLC)方法。研究结果表明,应用薄层层析技术对内生真菌的发酵产物进行检测比高效液相色谱(HPLC)方法更加高效,能够简便、准确的筛选到紫杉醇产生菌株,并且可以初步推测菌株的紫杉醇产生能力。更多还原

【Abstract】 In order to screen the taxol-producing fungus rapidly,the thin layer chromatography(TLC) method to detection of taxol was established.The result showed that the TLC detection was more efficient than high performance liquid chromatography on analysis of fermentation products of fungi.The taxol-producing fungus could be screened simply and accurately by TLC method,and the axol producing ability of fungus could be evaluated preliminarily.更多还原