【摘要】 HPLC方法测定额力根-7中羟基红花黄色素A含量。方法 :色谱柱C18(200×4.6 mm,5μm);流动相:甲醇-乙腈–磷酸-水(30:5:0.2:110);流速:1mL.min-1;测定波长:403nm。结果:羟基红花黄色素A在0.0025～0.2mg成线性关系(r=0.999);回收率为102.7%;RSD=2.9%;额力根-7中羟基红花黄色素A平均含量为1.24mg.g-1。结论:采用本法测定额力根-7中羟基红花黄色素A的含量,操作简便,准确可靠,可用于制定该药品的质量控制指标之一。更多还原

【Abstract】 Objective:To determinate Hydroxy safflower yellow A in Eligen-7 by means of HPLC.Method: C18 colum(200×4.6 mm,5μm)was used with the mobile phase of methano l-acetoneitrile-Phosphoric acid-water(30:5:0.2:110),at the detection wavelength of 403nm.The flow rate was 1mL·min-1,and the temperature of colum was 30℃.Results: Hydroxy safflower yellow A showed a good linear relation at a range of 0.0025～0.2mg,r=0.999.The recovery was 102.7% and RSD =2.9%,respectively.Conclusion :The method is simple,accurate and specifical.It can be used for determination of Hydroxy safflower yellow A in Eligen-7.更多还原