【摘要】 目的:检测sB7-H3在急性白血病病人脑脊液中的不同表达,以及分析该分子的表达与急性白血病的亚型的相关性。方法:采用ELISA的方法来检测sB7-H3在176位白血病病人脑脊液中的表达。依据国际公认的FAB(French-Amer-ican-British)分类系统将急性白血病病人分型。结果:sB7-H3在急性淋系白血病与急性髓系白血病中的表达差异显著(P=0.0381),这与人白血病细胞株的检测结果一致;在急性淋系白血病的亚型中,sB7-H3的表达没有显著性差异;在急性髓系白血病的亚型中,sB7-H3的表达在M3与M5和M4与M5之间存在显著性差异(分别为P=0.024 3和P=0.022 3)。结论:sB7-H3的表达与白血病的分型具有一定的相关性,同时也进一步证实B7-H3参与了人体免疫平衡的调节,可能会为今后急性白血病的临床诊断提供依据。更多还原

【Abstract】 Objective:To detect the different expression of soluble B7-H3(sB7-H3) in acute leukemia and analyze the association between the sB7-H3 level and the classification of acute leukemia.Methods:Enzyme-linked immunosorbent assay kits were used to measure the expression of sB7-H3 in cerebrospinal fluid(CSF) of 176 patients with acute leukemia.Classification of acute leukemia was accordance with the FAB(French-American-British) classification system.Results:There was signicant difference between acute lymphocyte leukemia(ALL) and acute myeloid leukemia(AML,P=0.038 1),which was the same as what in some human leukemia cell strains.However,there was no statistically signicant difference among the subtypes of ALL,but in the CSF of patients with AML,the level of sB7-H3 was significantly different between M3 and M5(P=0.024 3),M4 and M5(P=0.022 3).Conclusion:The level of sB7-H3 is associated with the classification of acute leukemia.B7-H3 has a role in the immune system balance.It may be useful in clinical diagnosis as a new technique.更多还原