【摘要】 目的检测正常孕妇妊娠不同阶段外周血单个核细胞(PBMC)巨噬细胞移动因子(MIF)基因表达水平及血清中MIF含量的变化,为揭示妊娠期间的调控机制提供实验依据。方法 42名妊娠不同阶段的正常孕妇来自天津市中医药大学第一附属医院,年龄26～36岁,平均(31.0±3.0)岁,其中妊娠早期18例,孕龄(8.71±1.35)周;妊娠中期9例,孕龄(21.35±3.37)周;妊娠晚期15例,孕龄(33.01±2.32)周。9例非妊娠对照组来自天津市血液中心健康女性志愿者,年龄(26.5±3.0)岁。用密度梯度离心法分离正常孕妇PBMC,调整细胞浓度为1×106个,提取RNA,反转录(RT)合成cDNA,用荧光定量聚合酶链反应(PCR)方法检测MIFmRNA表达水平,用酶联免疫吸附(ELISA)法检测孕妇血清中MIF含量。结果妊娠早期、中期、晚期PBMCMIF基因表达水平分别为2.15±0.47、2.07±0.39、2.18±0.54,与非妊娠对照组(0.68±0.12)比较,均有明显升高,差异有统计学意义(P<0.05)。妊娠早期、中期、晚期血清中MIF含量分别为(15.25±1.72)、(16.12±1.99)、(15.55±1.82)ng/ml,与非妊娠对照组([5.53±1.77)ng/ml]比较,均有明显升高,差异有统计学意义(P<0.05)。不同妊娠时间比较,MIFmRNA表达水平和MIF含量的差异均无统计学意义(P>0.05)。结论 MIF的基因表达和含量在整个妊娠过程中都维持在较高水平,提示MIF参与了整个妊娠至分娩。更多还原

【Abstract】 Objective To determine the mRNA expression of macrophage migration inhibitory factor(MIF) in peripheral blood mononuclear cells(PBMC) and its serum concentration in different pregnancy stages of normal pregnant women.Methods Forty-two normal pregnant wonen with different stages of pregnancy and 9 non-pregnant women as control were investigated.PBMC from subkects were isolated using density gradient centrifugation.The concentration of PBMC was adjusted to 1 ×10 6.Total RNA was extracted and then used for reverse transcription to synthesize cDNA.Real-time quantitative PCR was performed to determine the mRNA level of MIF.Serum MIF concentration was determined by ELISA.Results At the first pregnancy trimester,mid trimester,late trimester,the expression levels of MIF mRNA and serum MIF concentrations [2.15±0.47,2.07±0.39,2.18±0.54 and(15.25±1.72),(16.12±1.99),(15.55±1.82) ng/ml,respectively] were significantly higher than those [0.68 ±0.12,(5.53 ± 1.77) ng/ml] in control(all P<0.05).The expression levels of MIF mRNA and serum MIF concentrations in different pregnancy stages did not change significantly(P>0.05).Conclusion The expression levels of MIF mRNA and serum MIF concentrations remain a high level during the whole pregnancy.MIF may play an important role in pregnancy.更多还原