【摘要】 目的研究肠黏膜细胞内TLR9的活化对DSS诱导小鼠溃疡性结肠炎的影响.方法将小鼠随机分为6组,分别为PBS组、CpG ODN组、对照 ODN组、DSS+PBS组、DSS+CpG ODN组和DSS+对照 ODN组.其中PBS组、CpG ODN组、对照 ODN组的小鼠正常喂养,在当天给这3组小鼠通过腹腔分别注射PBS、CpG ODN和CpG ODN的对照 ODN;而给予DSS+PBS组、DSS+CpG ODN组和DSS对照 ODN组小鼠喂饲含有DSS的水,同样于当天给这3组小鼠通过腹腔分别注射PBS、CpG ODN和CpG ODN的对照 ODN.每天观察各组小鼠的状态、大便性状及大便隐血情况,作DAI评分;记录小鼠体质量变化.于第10天处死小鼠,分离结肠组织,进行组织学评分.结果经观察及检测发现:DSS+CpG ODN组小鼠的体质量下降明显,且状态不佳,DAI评分及组织学评分最高.结论 CpG ODN可加重DSS诱导的小鼠溃疡性结肠炎病情,对急性溃疡性结肠炎的造模具有促进作用.更多还原

【Abstract】 Objective To study the effect of activated TLR9 in intestinal epithelium on ulcerative colitis induced by DSS in mice.Method Mice were randomly divided into six groups:PBS group,CpG ODN group,control ODN group,DSS+PBS group,DSS+CpG ODN group and DSS+control ODN group.The mice in PBS group,CpG ODN group and control ODN group were fed normally and were injected intraperitoneally with PBS,CpG ODN,and control ODN,respectively.The mice in DSS+PBS group,DSS+CpG ODN group and DSS+control ODN group were fed with DSS in water for drinking and were injected intraperitoneally with PBS,CpG ODN,and control ODN,respectively.Disease active index(DAI)was recorded every day.After ten days the mice were killed and their colons were taken out to undergo histological examination.Results The loss of weight in DSS+CpG ODN group was more than those in other groups(P<0.05).Moreover,the DAI score and histological score in the group of DSS+CpG ODN were significantly higher than those in other groups(P<0.05).Conclusion The CpG ODN could aggravate the ulcerative colitis induced by DSS in mice.更多还原