【摘要】 目的探讨氧化高密度脂蛋白(oxHDL)及其亚型对人脐静脉内皮细胞(HUVECs)组织因子途径抑制物-1(TFPI-1)表达的影响。方法以天然HDL及其亚型作为对照,HUVECs分别经不同浓度(0,10,20,40,80 mg/L)oxHDL、oxHDL2、oxHDL3孵育24 h,逆转录聚合酶链反应(RT-PCR)、Western印迹分别检测TFPI-1 mRNA及蛋白质表达。结果与对照组比较,oxHDL、oxHDL2、oxHDL3能抑制TFPI-1 mRNA和蛋白表达,其中在oxHDL和oxHDL3各系列浓度中以40 mg/L作用最明显,TFPI-1 mRNA和蛋白表达分别下降了28%、35%和49%、47%;在oxHDL2各系列浓度中以80 mg/L作用最明显,TFPI-1 mRNA和蛋白表达均下降了27%,差异均具有统计学意义(P<0.05);且以oxHDL3的作用最强。结论 oxHDL及其亚型能抑制HUVECs TFPI-1 mRNA和蛋白表达,oxHDL3的作用最强。更多还原

【Abstract】 Objective To investigate the expression of tissue factor pathway inhibitor-1(TFPI-1) induced by oxidized high densitive lipoprotein(HDL) and its subfractions in human umbilical vein endothelial cells(HUVECs).Methods HUVECs were incubated with oxHDL,oxHDL2,oxHDL3 or their native forms at different concentrations(0,10,20,40,80 mg/L)for 24 h.TFPI-1 mRNA and protein expressions were determined by RT-PCR and Western blot,respectively.Results Compared with control group(ie.HDL,HDL2 or HDL3),oxHDL,oxHDL2,oxHDL3 significantly decreased the expressions of TFPI-1 mRNA and protein,respectively.TFPI-1 mRNA and protein expressions were lowered by oxHDL by 28% and 35%,respectively at the level of 40 mg/L,by oxHDL2 by 27% and 27% at the level of 80 mg/L,and by oxHDL3 by 49% and 47%,respectively,at the level of 40 mg/L(all P<0.05).The effect of oxHDL3 appeared the strongest.Conclusions Ox-HDL and its subfractions could inhibit the expression levels of TFPI-1 mRNA and protein in HUVECs.更多还原