【摘要】 目的探讨亚甲蓝(MB)对APP/PS1转基因小鼠海马CA1区树突棘密度改变及学习记忆能力改善的影响。方法 APP/PS1小鼠及相同品系的3月龄野生小鼠,随机分为3组,每组10只:正常对照组,为野生小鼠。模型组,为APP/PS1小鼠;治疗组,3月龄的APP/PS1小鼠口服亚甲蓝25 mg·kg-1·d-1;连续用药4个月。待3组小鼠均为7月龄时,跳台实验测试3组小鼠的学习记忆能力;高尔基染色观察各组海马CA1区树突棘密度的变化。结果亚甲蓝治疗组小鼠跳台试验错误次数减少,小鼠跳台试验的潜伏期明显延长(P<0.01);治疗组与模型组相比海马CA1区树突棘密度明显增加(P<0.01),治疗组与对照组无统计学差异(P>0.05)。结论亚甲蓝可能是通过增加海马CA1区树突棘密度来改善APP/PS1小鼠的学习记忆能力。更多还原

【Abstract】 Objective To investigate the effect of methylene blue(MB) on the ability of learning and memorizing and figure out the density of dendritic spine in hippocampal CA1 area of APP/PS1 transgenic mice.Methods 3-month-old APP/PS1 mice and wide type(WT) mice were divided into MB treated(treated with MB 25 mg·kg-1·d-1,p.o.),model(without MB treatment),and normal control groups(WT mice).The ability of learning and memorizing were assessed by step-down avoidance test 4 months later.The density of dendritic spine was observed by Golgi staining.Results Step-down avoidance test showed a decrease in the number of errors and a prolongation in latency(P<0.01) after MB treatment.Compared with model group,the density of dendritic spine in MB-treated group was significantly increased.There was no statistical difference between model and MB-treated groups(P<0.05).Conclusions MB could improve the ability of learning and memorizing by increasing the density of dendritic spine.更多还原