【摘要】 目的:探讨中药香加皮提取物三萜类化合物(triterpenes compound of cortex periplocae,TCCP)对甲基苄基亚硝胺(N-nitrosomethylbenzylamine,NMBA)诱导的大鼠食管癌组织中增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)表达的影响。方法:雄性F344大鼠120只,随机分为NMBA模型组、TCCP干预组、大豆油对照组和正常对照组。模型组大鼠皮下注射NMBA,TCCP干预组大鼠同时给予皮下注射NMBA及肌注TCCP,大豆油对照组大鼠肌注大豆油,正常对照组大鼠常规饲养。分别在给药后第9、15和25周,H-E染色检测大鼠食管上皮组织病理变化,免疫组织化学SP法检测大鼠食管组织中PCNA的表达。结果:正常对照组及大豆油对照组大鼠食管未发现异常变化,NMBA模型组大鼠9周时,食管癌前病变发生率为20.0%,15周时为46.7%,25周时达93.3%。与NMBA模型组相比,第9、15周时TCCP干预组癌前病变大鼠的比例明显降低(0、0 vs 20.0%、46.7%,P<0.05)。NMBA模型组第9、15和25周时,大鼠食管上皮组织PCNA表达水平显著高于正常对照组(213.17±29.74 vs 167.96±20.16,268.35±39.56 vs 170.76±14.79,327.24±28.19 vs 172.49±17.49;P<0.05);与NMBA模型组相比,TCCP干预组大鼠食管上皮组织中PCNA表达水平显著降低(P<0.05)。结论:TCCP可抑制NMBA诱导的大鼠食管癌前病变,该作用可能与其抑制PCNA的表达有关。更多还原

【Abstract】 Objective: To investigate the effect of triterpenes compound of cortex periplocae(TCCP) on the expression of proliferating cell nuclear antigen(PCNA) in N-nitrosomethylbenzylamine(NMBA)-induced rat esophageal carcinoma.Methods: 120 male F344 rats were randomly divided into four groups: a model group treated with NMBA only and a TCCP treatment group receiving NMBA plus TCCP,a soya oil control group treated with soya oil and a normal control group.At 9,15 and 25 weeks after treatment,the pathological changes of esophageal tissues were detected by H-E staining,while the expression of PCNA was measured by immunohistochemistry.Results: There were no abnormal changes in the normal and soya oil groups.At 9,15 and 25 weeks after treatment,the incidence of preneoplastic lesion in the NMBA group was 20.0%,46.7% and 93.3%,respectively.At 9 and 15 weeks,the rate of rat esophageal preneoplastic lesion significantly decreased in TCCP treatment group compared with NMBA group(0,0 vs 20.0%,46.7%,P<0.05).There was a significant increase in the expression of PCNA in the NMBA group(week 9: 213.17±29.74;week 15: 268.35±39.56;week 25: 327.24±28.19),compared with that in the normal control group([167.96±20.16],[170.76±14.79],[172.49±17.49],P<0.05).TCCP significantly decreased the expression of PCNA compared with that in the NMBA group(P<0.05).Conclusion: TCCP inhibits NMBA-induced rat esophageal carcinoma probably via suppression of PCNA expression.更多还原