【摘要】 目的观察心肌肥厚大鼠心肌组织中整合素β1的表达变化。方法在大鼠背部皮下分别注射7 d、28 d和56 d异丙肾上腺素(ISO),判定心肌肥厚指标,利用免疫组织化学和免疫印迹技术,观察整合素β1在肥厚左心室肌中的表达。结果免疫组化和Western blot结果显示,整合素β1主要表达于心肌细胞膜。与对照组相比,实验各组大鼠的心肌组织的整合素β1表达明显(P<0.05)。7 d、28 d和56 d各组间相比其表达含量逐渐增强。结论在心肌肥厚状态下,心肌细胞中的整合素β1表达增高,随着心肌肥大的进展其表达逐渐增加,提示整合素β1在心肌肥厚发生发展起重要作用。更多还原

【Abstract】 Objective To explore the expression changes of the integrin β1 in hypertrophic rat myocardium.Methods The rat models of myocardial hypertrophy were prepared by isoprenaline(ISO) injection into dorsal subcutis at 7d,28d and 56d respectively.Myocardial hypertrophic indexes of model animals were assessed.Immunohistochemisty and western blot were used to investigate integrin β1 expression in left ventricular tissue.Results Integrin β1 presented mainly in myocardial membrane.The expression of integrinβ1 in hypertrophic myocardium was higher than that of control group(P<0.05),furthermore,the expression level increased gradually from 7d,28d to 56d.Conclusions Along the development of cardiac hypertrophy,the expression level of integrin β1 increases gradually,which demonstrates that integrin β1 plays an important role during the development of myocardial hypertrophy.更多还原