【摘要】 从垂序商陆(Phytolacca americana)的重金属镉胁迫下商陆幼苗叶片的正反向抑制性消减杂交文库(SSH文库)中,获得商陆寡肽转运蛋白基因的EST序列.利用RACE的方法克隆到该基因的全序列cDNA(PaOPT,GenBank注册HQ647015).PaOPT基因在进化中的地位分析表明:它可能属于OPT3基因家族.利用实时荧光定量PCR的方法,分析PaOPT的组织表达特异性以及商陆种子成熟及萌发过程的表达模式.结果表明,该基因是一个在种子中特异性表达的基因,预测它对植物的生长发育有着重要的作用.更多还原

【Abstract】 An oligopeptide transporter gene was isolated from Cd-treated leaf tissue of Phytolacca americana by SSH method,designated by PaOPT(GenBank accession No.HQ647015).The full length of this gene has been cloned by race-PCR technique.Moreover,the evolution of PaOPT in OPT family and the expression profiles during seed germination period was investigated.Our study indicates that PaOPT belongs to the OPT3 family.The results of real-time quantitative PCR show that PaOPT may be a seed-specific expression gene.Further investigation of the function and localization of PaOPT will be favorable for understanding of plant growth and development.更多还原