【摘要】 目的:分析重性抑郁障碍患者(Major depressive disorder,MDD)血浆兴奋性氨基酸类神经递质天冬氨酸(Aspartic acid,Asp)和抑制性神经递质甘氨酸(Glycine,Gly),以及氨基酸天冬酰胺(Asparagine,Asn)水平的变化。方法:收集15例首发重性抑郁障碍患者(男9例,女6例,年龄32～64岁)和14例健康对照者(男7例,女7例,年龄30～65岁)的血浆,其中7例MDD患者(男5例,女2例)在抗抑郁治疗两月后的随访中再次留取血浆。采用高效液相色谱—荧光法测定血浆中Asp、Gly、Asn含量。结果:(1)MDD患者血浆Asp、Gly水平均显著低于健康对照组(P≤0.033),Asn水平和健康对照组相比无显著差异(P=0.208)。(2)在健康对照组,血浆Gly与Asp、Asn水平分别呈现显著正相关(P≤0.004)。在MDD患者,血浆Gly水平与Asp水平的相关性消失(P=0.538),而Gly水平和Asn水平保持相关性(P<0.001)。(3)抗抑郁治疗两个月后MDD患者临床症状显著改善,血浆Asp、Gly、Asn水平和治疗前相比无显著差异(P≥0.306),氨基酸水平之间的相关性也无改变。结论:血浆Asp和Gly水平降低可能是MDD的一个特征性生物学指标。更多还原

【Abstract】 Objective: To evaluate the changes of plasma levels of the excitatory amino acid neurotransmitter aspartic acid(Asp),inhibitory neurotransmitter glycine(Gly) and asparagine(Asn) in patients with major depressive disorder(MDD).Methods: Plasma samples were collected from 15 MDD patients(9 males and 6 females,aged 32-64 y) and 14 healthy subjects(7 males and 7 females,aged 30-65 y);and also collected from 7 MDD patients(5 males and 2 females) 2 months after antidepressant treatment.The plasma levels of amino acids were determined by high performance liquid chromatography with fluorescence detection method.Results: Plasma Asp and Gly levels were significantly lower in MDD patients than those in controls(P≤0.033).There were positive correlations between plasma levels of Gly and Asp,and between Gly and Asn(P≤0.004) in the control group;while in MDD patients,a significant positive correlation was found only between plasma levels of Gly and of Asp(P<0.001).MDD patients did not show significant changes in plasma Asp,Asn and Gly levels after antidepressant treatment compared to those before treatment.Conclusion: The reduced plasma Asp and Gly levels may serve as a clinical biomarker for MDD.更多还原