【摘要】 [目的]研究大剂量甲强龙(糖皮质激素)对股骨头、股骨近端组织BMP-2表达的影响,探索ONFH行人工关节置换治疗翻修率较高的原因。[方法]健康新西兰成年大白兔45只,随机分为1个对照组(A组),2个实验组(B、C组),每组均15只,B组连续3 d,C组连续6 d肌注甲强龙80 mg/(kg.d),诱发股骨头坏死模型,分别于注射后2、4、8周切取双侧股骨头、小转子处约3 mm厚的股骨近端段,行SABC法免疫组化染色,观察BMP-2表达情况。[结果]对于股骨头、股骨近端骨,激素使用剂量或作用时间,均可致BMP-2的表达减少,且激素使用剂量与作用时间有交互作用,(P<0.05)。[结论]大剂量甲强龙可以造成股骨头、股骨近端骨组织内BMP-2表达降低,激素的应用时间越长,应用总量越大,对其骨骼的影响越严重。更多还原

【Abstract】 [Objective]To study the effects of high dosage of methylprednisolone on acetabulum,caput ossis femoris and proximal femurs in a rabbit model by expression of BMP-2.[Method]Twenty-seven adult rabbits were divided into three groups.Besides control group(group A),experimental groups were consisted group B and group C,with 9 rabbits in each group.The animal model of osteonecrosis of the femoral head(ONFH) was established by intramuscular injection of methylprednisolone sodium succinate 80 mg/kg/d in rabbits,for 3 days in group B,for 6 days in group C.At 2,4,8 weeks after steroid administration,the bone tissue of caput ossis femoris and proximal femurs were collected and the changes of expression of BMP-2 were observed.[Result]The average percentage of dyed areas and average optical density of BMP-2 had a trend of decreasing with time and dosages increased in group B,C.No dyed areas was observed in individual samples(P<0.05).[Conclusion]High dosages of methylprednisolone can lead to osteonecrosis of femoral head and proximal femurs,reduce the expression of BMP-2.The higher the dosages of methylprednisolone are administered and the longer the administering time lasts,the poorer of the bone of hip and the proximal femur will be.更多还原